An integrated nano-scale approach to profile miRNAs in
limited clinical samples
An integrated nano-scale approach to profile miRNAs in
limited clinical samples
Profiling miRNA expression in cells that directly contribute to human disease pathogenesis is likely to aid the discovery of novel drug targets and biomarkers. However, tissue heterogeneity and the limited amount of human diseased tissue available for research purposes present fundamental difficulties that often constrain the scope and potential of such studies. We established a flow cytometry-based method for isolating pure populations of pathogenic T cells from bronchial biopsy samples of asthma patients, and optimized a high-throughput nano-scale qRT-PCR method capable of accurately measuring 96 miRNAs in as little as 100 cells. Comparison of circulating and airway T cells from healthy and asthmatic subjects revealed asthma associated and tissue-specific miRNA expression patterns. These results establish the feasibility and utility of investigating miRNA expression in small populations of cells involved in asthma pathogenesis, and set a precedent for application of our nano-scale approach in other human diseases. The microarray data from this study (Figure 7) has been submitted to the NCBI Gene Expression Omnibus (GEO; http://ncbi.nlm.nih.gov/geo) under accession no. GSE31030.
microrna (mirna), asthma, helper t cell, microfluidic, qpcr arrays
70-89
Seumois, Grégory
0be7d3d6-5526-458c-aa5c-cce52410a2ed
Vijayanand, Pandurangan
79514f33-66cf-47cc-a8fa-46bbfc21b7d1
Eisley, Christopher J.
f1c67db7-bae5-45fa-b6d2-2435de92afed
Omran, Nada
cde20d0c-fd4b-4f9e-8ee2-30a74f310e16
Kalinke, Lukas
841230f7-4bd8-4c69-8133-628702ef8956
North, Mal
9d5d5ba9-7534-4959-a9a1-298f5a2d0db7
Ganesan, Asha P.
41f5f461-cfeb-4bfd-967b-aa3adf5f9df8
Simpson, Laura J.
4cc2b77c-d8e8-4379-97b8-1cae8f5f34c2
Hunkapiller, Nathan
94943c9e-183e-4444-8161-4124db7a24b9
Moltzahn, Felix
a9707583-933e-4f47-a30b-7ceb19f30e14
Woodruff, Prescott G.
02887f00-a7bb-459e-8c79-08bd91354a9b
Fahy, John V.
28d57b70-d25e-4484-9015-c9ba2bcd0505
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Blelloch, Robert
4dae5fcf-4589-4a2c-bc1f-c9f8053bed42
Ansel, K. Mark
419a9477-acf1-411b-b9d4-a9504bb18bce
Erle, David J.
2befd4eb-9cdf-47f8-aa78-8e635e6e2170
30 November 2012
Seumois, Grégory
0be7d3d6-5526-458c-aa5c-cce52410a2ed
Vijayanand, Pandurangan
79514f33-66cf-47cc-a8fa-46bbfc21b7d1
Eisley, Christopher J.
f1c67db7-bae5-45fa-b6d2-2435de92afed
Omran, Nada
cde20d0c-fd4b-4f9e-8ee2-30a74f310e16
Kalinke, Lukas
841230f7-4bd8-4c69-8133-628702ef8956
North, Mal
9d5d5ba9-7534-4959-a9a1-298f5a2d0db7
Ganesan, Asha P.
41f5f461-cfeb-4bfd-967b-aa3adf5f9df8
Simpson, Laura J.
4cc2b77c-d8e8-4379-97b8-1cae8f5f34c2
Hunkapiller, Nathan
94943c9e-183e-4444-8161-4124db7a24b9
Moltzahn, Felix
a9707583-933e-4f47-a30b-7ceb19f30e14
Woodruff, Prescott G.
02887f00-a7bb-459e-8c79-08bd91354a9b
Fahy, John V.
28d57b70-d25e-4484-9015-c9ba2bcd0505
Erle, David J.
2befd4eb-9cdf-47f8-aa78-8e635e6e2170
Djukanovic, Ratko
d9a45ee7-6a80-4d84-a0ed-10962660a98d
Blelloch, Robert
4dae5fcf-4589-4a2c-bc1f-c9f8053bed42
Ansel, K. Mark
419a9477-acf1-411b-b9d4-a9504bb18bce
Seumois, Grégory, Vijayanand, Pandurangan, Eisley, Christopher J., Omran, Nada, Kalinke, Lukas, North, Mal, Ganesan, Asha P., Simpson, Laura J., Hunkapiller, Nathan, Moltzahn, Felix, Woodruff, Prescott G., Fahy, John V., Djukanovic, Ratko, Blelloch, Robert and Ansel, K. Mark
,
Erle, David J.
(ed.)
(2012)
An integrated nano-scale approach to profile miRNAs in
limited clinical samples.
American Journal of Clinical and Experimental Immunology, 1 (2), .
(PMID:23304658)
Abstract
Profiling miRNA expression in cells that directly contribute to human disease pathogenesis is likely to aid the discovery of novel drug targets and biomarkers. However, tissue heterogeneity and the limited amount of human diseased tissue available for research purposes present fundamental difficulties that often constrain the scope and potential of such studies. We established a flow cytometry-based method for isolating pure populations of pathogenic T cells from bronchial biopsy samples of asthma patients, and optimized a high-throughput nano-scale qRT-PCR method capable of accurately measuring 96 miRNAs in as little as 100 cells. Comparison of circulating and airway T cells from healthy and asthmatic subjects revealed asthma associated and tissue-specific miRNA expression patterns. These results establish the feasibility and utility of investigating miRNA expression in small populations of cells involved in asthma pathogenesis, and set a precedent for application of our nano-scale approach in other human diseases. The microarray data from this study (Figure 7) has been submitted to the NCBI Gene Expression Omnibus (GEO; http://ncbi.nlm.nih.gov/geo) under accession no. GSE31030.
Text
__userfiles.soton.ac.uk_Users_bct_mydocuments_REF_Final submission - Validation errors_Output pdfs_Eprint349173-ajcei2012,1,70-89.pdf
- Version of Record
Restricted to Repository staff only
Request a copy
More information
e-pub ahead of print date: 27 September 2012
Published date: 30 November 2012
Keywords:
microrna (mirna), asthma, helper t cell, microfluidic, qpcr arrays
Organisations:
Clinical & Experimental Sciences
Identifiers
Local EPrints ID: 349173
URI: http://eprints.soton.ac.uk/id/eprint/349173
PURE UUID: c2185885-3bfe-4144-b5c3-dabedc12c8c5
Catalogue record
Date deposited: 27 Feb 2013 09:56
Last modified: 15 Mar 2024 02:36
Export record
Contributors
Author:
Grégory Seumois
Author:
Pandurangan Vijayanand
Author:
Christopher J. Eisley
Author:
Nada Omran
Author:
Lukas Kalinke
Author:
Mal North
Author:
Asha P. Ganesan
Author:
Laura J. Simpson
Author:
Nathan Hunkapiller
Author:
Felix Moltzahn
Author:
Prescott G. Woodruff
Author:
John V. Fahy
Editor:
David J. Erle
Author:
Robert Blelloch
Author:
K. Mark Ansel
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics