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Monocytes and plasma tissue factor levels in normal individuals and patients with deep venous thrombosis of the lower limbs: potential diagnostic tools?

Monocytes and plasma tissue factor levels in normal individuals and patients with deep venous thrombosis of the lower limbs: potential diagnostic tools?
Monocytes and plasma tissue factor levels in normal individuals and patients with deep venous thrombosis of the lower limbs: potential diagnostic tools?
INTRODUCTION: Tissue factor (TF) is the main physiological initiator of blood coagulation; it is membrane-bound on monocytes (mTF) and free in plasma (pTF). Abnormal expression of TF by monocytes has been implicated in various diseases. We therefore quantified monocytes expressing TF and pTF levels in patients with lower-limb deep venous thrombosis (DVT).

MATERIALS AND METHODS: DVT was confirmed by Duplex Scan. Blood mTF levels under resting condition (baseline), after incubation without (unstimulated) and with (stimulated) lipopolysaccharide (LPS), and total mTF levels were determined by flow cytometry using two analytical methods (Histogram and Quadrant-Statistics). Plasma TF levels were measured using an enzyme-linked immunoabsorbent assay (ELISA). Results were compared with age-matched controls.

RESULTS: Histogram analysis in patients with DVT showed significantly elevated mTF levels for baseline, unstimulated and total mTF over controls. For Quadrant-Statistics, DVT patients also showed significantly raised baseline, unstimulated, stimulated and total mTF. Similarly, pTF levels were significantly raised in subjects with DVT compared to controls. Baseline mTF levels correlated with pTF levels by Histogram and Quadrant-Statistics analysis. Using the relative operating characteristic (ROC) curve, baseline mTF and pTF assays displayed sensitivity and specificity in detecting DVT. Quadrant-Statistics baseline mTF and pTF gave the best discrimination.

CONCLUSIONS: The TF assays used in this study showed acceptable sensitivity and specificity and are cost-effective and practical. Therefore, they should be considered in patients with, or at risk of, DVT.
deep venous thrombosis (DVT), monocytes, endothelial cells, plasma, tissue factor, flow cytometry, ELISA, diagnosis, original article
0049-3848
157-165
Vieira, Lauro M.
ae7e87cf-8dd3-4a36-87f0-6fc053562e8a
Dusse, Luci M.S.
b9464ddb-7589-4425-9856-3ba9f07a4c59
Fernandes, Ana P.
f3e4e2c3-ef81-44a2-8c5c-b41067ad7585
Martins-Filho, Olindo A.
efe9698f-ed2a-4d8e-b6cd-76d9e6911c00
de Bastos, Marcos
85261c01-72e5-4d80-a403-262cbc6ceb8c
Ferreira, Mônica F.R.
14e79b7d-9369-494d-91ac-e364fe12acab
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Carvalho, Maria G.
b7495b77-148e-47f5-88f2-f613b58be29f
Vieira, Lauro M.
ae7e87cf-8dd3-4a36-87f0-6fc053562e8a
Dusse, Luci M.S.
b9464ddb-7589-4425-9856-3ba9f07a4c59
Fernandes, Ana P.
f3e4e2c3-ef81-44a2-8c5c-b41067ad7585
Martins-Filho, Olindo A.
efe9698f-ed2a-4d8e-b6cd-76d9e6911c00
de Bastos, Marcos
85261c01-72e5-4d80-a403-262cbc6ceb8c
Ferreira, Mônica F.R.
14e79b7d-9369-494d-91ac-e364fe12acab
Cooper, Alan J.
65dcd1e1-3fcd-46b8-ad5f-f17e0d5b80a5
Lwaleed, Bashir A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Carvalho, Maria G.
b7495b77-148e-47f5-88f2-f613b58be29f

Vieira, Lauro M., Dusse, Luci M.S., Fernandes, Ana P., Martins-Filho, Olindo A., de Bastos, Marcos, Ferreira, Mônica F.R., Cooper, Alan J., Lwaleed, Bashir A. and Carvalho, Maria G. (2007) Monocytes and plasma tissue factor levels in normal individuals and patients with deep venous thrombosis of the lower limbs: potential diagnostic tools? Thrombosis Research, 119 (2), 157-165. (doi:10.1016/j.thromres.2006.01.005). (PMID:16527335)

Record type: Article

Abstract

INTRODUCTION: Tissue factor (TF) is the main physiological initiator of blood coagulation; it is membrane-bound on monocytes (mTF) and free in plasma (pTF). Abnormal expression of TF by monocytes has been implicated in various diseases. We therefore quantified monocytes expressing TF and pTF levels in patients with lower-limb deep venous thrombosis (DVT).

MATERIALS AND METHODS: DVT was confirmed by Duplex Scan. Blood mTF levels under resting condition (baseline), after incubation without (unstimulated) and with (stimulated) lipopolysaccharide (LPS), and total mTF levels were determined by flow cytometry using two analytical methods (Histogram and Quadrant-Statistics). Plasma TF levels were measured using an enzyme-linked immunoabsorbent assay (ELISA). Results were compared with age-matched controls.

RESULTS: Histogram analysis in patients with DVT showed significantly elevated mTF levels for baseline, unstimulated and total mTF over controls. For Quadrant-Statistics, DVT patients also showed significantly raised baseline, unstimulated, stimulated and total mTF. Similarly, pTF levels were significantly raised in subjects with DVT compared to controls. Baseline mTF levels correlated with pTF levels by Histogram and Quadrant-Statistics analysis. Using the relative operating characteristic (ROC) curve, baseline mTF and pTF assays displayed sensitivity and specificity in detecting DVT. Quadrant-Statistics baseline mTF and pTF gave the best discrimination.

CONCLUSIONS: The TF assays used in this study showed acceptable sensitivity and specificity and are cost-effective and practical. Therefore, they should be considered in patients with, or at risk of, DVT.

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More information

e-pub ahead of print date: 9 March 2006
Published date: 2007
Keywords: deep venous thrombosis (DVT), monocytes, endothelial cells, plasma, tissue factor, flow cytometry, ELISA, diagnosis, original article
Organisations: Faculty of Health Sciences

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Local EPrints ID: 349255
URI: http://eprints.soton.ac.uk/id/eprint/349255
ISSN: 0049-3848
PURE UUID: 623e3c9e-5837-4a12-b22c-154d4d3daa41

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Date deposited: 26 Feb 2013 16:46
Last modified: 16 Jul 2019 21:42

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Contributors

Author: Lauro M. Vieira
Author: Luci M.S. Dusse
Author: Ana P. Fernandes
Author: Olindo A. Martins-Filho
Author: Marcos de Bastos
Author: Mônica F.R. Ferreira
Author: Alan J. Cooper
Author: Maria G. Carvalho

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