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Development and validation of an assay for urinary tissue factor activity

Development and validation of an assay for urinary tissue factor activity
Development and validation of an assay for urinary tissue factor activity
BACKGROUND: Activation of blood coagulation is a common complication of cancer and inflammation in both humans and experimental animals. Increased production of tissue factor--the principal initiator of the coagulation process--by endothelial cells, monocytes, and macrophages has been implicated in these conditions.

AIM: To investigate whether urinary tissue factor (uTF) might reflect the state of monocyte/macrophage activation and be a useful diagnostic test.

METHODS: Urine was centrifuged at 51,000 g to sediment tissue factor containing membrane vesicles. The tissue factor was then solubilised in beta-octyl-glucopyranoside and assayed in a specific chromogenic assay adapted for use in microtitre plates.

RESULTS: The assay proved to be sensitive, specific, and reproducible. The normal range of uTF was relatively narrow and unaffected by age, sex, or cigarette smoking. Levels were not significantly influenced by storage of urine samples before assay or by the presence of fresh blood in the urine sample.

CONCLUSIONS: This method may have diagnostic application in the study of haemostatic activation in patients with cancer and other disease states.
0021-9746
219-224
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Chisholm, M.
c45aab85-a073-4041-9d3f-f7cd738aae4f
Francis, J.L.
54a1f2e1-fd7a-4592-b55c-3c856810b68d
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Chisholm, M.
c45aab85-a073-4041-9d3f-f7cd738aae4f
Francis, J.L.
54a1f2e1-fd7a-4592-b55c-3c856810b68d

Lwaleed, B.A., Chisholm, M. and Francis, J.L. (1999) Development and validation of an assay for urinary tissue factor activity. Journal of Clinical Pathology, 52 (3), 219-224. (doi:10.1136/jcp.52.3.219). (PMID:10450183)

Record type: Article

Abstract

BACKGROUND: Activation of blood coagulation is a common complication of cancer and inflammation in both humans and experimental animals. Increased production of tissue factor--the principal initiator of the coagulation process--by endothelial cells, monocytes, and macrophages has been implicated in these conditions.

AIM: To investigate whether urinary tissue factor (uTF) might reflect the state of monocyte/macrophage activation and be a useful diagnostic test.

METHODS: Urine was centrifuged at 51,000 g to sediment tissue factor containing membrane vesicles. The tissue factor was then solubilised in beta-octyl-glucopyranoside and assayed in a specific chromogenic assay adapted for use in microtitre plates.

RESULTS: The assay proved to be sensitive, specific, and reproducible. The normal range of uTF was relatively narrow and unaffected by age, sex, or cigarette smoking. Levels were not significantly influenced by storage of urine samples before assay or by the presence of fresh blood in the urine sample.

CONCLUSIONS: This method may have diagnostic application in the study of haemostatic activation in patients with cancer and other disease states.

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Published date: March 1999
Organisations: Faculty of Health Sciences

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Local EPrints ID: 349267
URI: http://eprints.soton.ac.uk/id/eprint/349267
ISSN: 0021-9746
PURE UUID: a85bfecb-00e3-46f0-b18c-214937dca05d

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Date deposited: 27 Feb 2013 12:25
Last modified: 16 Jul 2019 21:42

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Contributors

Author: B.A. Lwaleed
Author: M. Chisholm
Author: J.L. Francis

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