The University of Southampton
University of Southampton Institutional Repository

Functional and structural properties of urinary tissue factor

Functional and structural properties of urinary tissue factor
Functional and structural properties of urinary tissue factor
BACKGROUND: We have previously explored the clinical significance of urinary tissue factor (uTF) in patients with glomerulonephritis (GN) and malignancy. However, the functional and structural properties and putative cell of origin of uTF are poorly documented. In these studies we investigate these aspects of uTF.

METHODS: Functional and structural properties of uTF were investigated using a one stage kinetic chromogenic assay, an enzyme-linked immunoabsorbent assay (ELISA) and transmission electron microscopy (TEM) on urine samples collected from healthy controls (n=69). The distribution of uTF and anionic phospholipid in the kidney were studied in sections from normal areas of nephrectomy specimens, using an immunoperoxidase technique. These were stained for tissue factor (TF) antigen and recombinant Annexin V.

RESULTS: We found uTF to be present on subcellular particles as visualized by TEM. These particles contained anionic phospholipid as evidenced by binding to Annexin V fluorescein isothiocyanate (FITC). Although TF is present in urine in a functional and antigenic form no association was observed between the two. Using immunoperoxidase-based techniques, the cytoplasm of both distal and proximal tubules (but not glomerular cells) was positive for TF antigen and Annexin V.

CONCLUSION: uTF is found on subcellular particles which provide lipid for its functional activity. Both uTF and its associated vesicles are found in the renal tubular cells.
0931-0509
588-596
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Bass, P.S.
8498593e-3539-4908-80da-e2606153869a
Francis, J.L.
54a1f2e1-fd7a-4592-b55c-3c856810b68d
Chisholm, M.
c45aab85-a073-4041-9d3f-f7cd738aae4f
Lwaleed, B.A.
e7c59131-82ad-4a14-a227-7370e91e3f21
Bass, P.S.
8498593e-3539-4908-80da-e2606153869a
Francis, J.L.
54a1f2e1-fd7a-4592-b55c-3c856810b68d
Chisholm, M.
c45aab85-a073-4041-9d3f-f7cd738aae4f

Lwaleed, B.A., Bass, P.S., Francis, J.L. and Chisholm, M. (1999) Functional and structural properties of urinary tissue factor. Nephrology, Dialysis, Transplantation, 14 (3), 588-596. (PMID:10193804)

Record type: Article

Abstract

BACKGROUND: We have previously explored the clinical significance of urinary tissue factor (uTF) in patients with glomerulonephritis (GN) and malignancy. However, the functional and structural properties and putative cell of origin of uTF are poorly documented. In these studies we investigate these aspects of uTF.

METHODS: Functional and structural properties of uTF were investigated using a one stage kinetic chromogenic assay, an enzyme-linked immunoabsorbent assay (ELISA) and transmission electron microscopy (TEM) on urine samples collected from healthy controls (n=69). The distribution of uTF and anionic phospholipid in the kidney were studied in sections from normal areas of nephrectomy specimens, using an immunoperoxidase technique. These were stained for tissue factor (TF) antigen and recombinant Annexin V.

RESULTS: We found uTF to be present on subcellular particles as visualized by TEM. These particles contained anionic phospholipid as evidenced by binding to Annexin V fluorescein isothiocyanate (FITC). Although TF is present in urine in a functional and antigenic form no association was observed between the two. Using immunoperoxidase-based techniques, the cytoplasm of both distal and proximal tubules (but not glomerular cells) was positive for TF antigen and Annexin V.

CONCLUSION: uTF is found on subcellular particles which provide lipid for its functional activity. Both uTF and its associated vesicles are found in the renal tubular cells.

Full text not available from this repository.

More information

Published date: March 1999
Organisations: Faculty of Health Sciences

Identifiers

Local EPrints ID: 349271
URI: http://eprints.soton.ac.uk/id/eprint/349271
ISSN: 0931-0509
PURE UUID: 963a3b9e-2c9c-44aa-a8c7-620a6a8a0d2d

Catalogue record

Date deposited: 27 Feb 2013 13:08
Last modified: 16 Jul 2019 21:42

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×