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Efficient self-assembly of DNA-functionalized fluorophores and gold nanoparticles with DNA functionalized silicon surfaces: the effect of oligomer spacers

Efficient self-assembly of DNA-functionalized fluorophores and gold nanoparticles with DNA functionalized silicon surfaces: the effect of oligomer spacers
Efficient self-assembly of DNA-functionalized fluorophores and gold nanoparticles with DNA functionalized silicon surfaces: the effect of oligomer spacers
Although strategies for the immobilization of DNA oligonucleotides onto surfaces for bioanalytical and top-down bio-inspired nanobiofabrication approaches are well developed, the effect of introducing spacer molecules between the surface and the DNA oligonucleotide for the hybridization of nanoparticle–DNA conjugates has not been previously assessed in a quantitative manner. The hybridization efficiency of DNA oligonucleotides end-labelled with gold nanoparticles (1.4 or 10 nm diameter) with DNA sequences conjugated to silicon surfaces via hexaethylene glycol phosphate diester oligomer spacers (0, 1, 2, 6 oligomers) was found to be independent of spacer length. To quantify both the density of DNA strands attached to the surfaces and hybridization with the surface-attached DNA, new methodologies have been developed. Firstly, a simple approach based on fluorescence has been developed for determination of the immobilization density of DNA oligonucleotides. Secondly, an approach using mass spectrometry has been created to establish (i) the mean number of DNA oligonucleotides attached to the gold nanoparticles and (ii) the hybridization density of nanoparticle–oligonucleotide conjugates with the silicon surface–attached complementary sequence. These methods and results will be useful for application with nanosensors, the self-assembly of nanoelectronic devices and the attachment of nanoparticles to biomolecules for single-molecule biophysical studies.
0305-1048
e80
Milton, James A.
9e183221-d0d4-4ddb-aeba-0fdde9d31230
Patole, Samson
c35e3cba-1913-4a6a-a78e-942495470f6e
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Xiao, Qiang
abe7be8e-6131-403e-aa22-2fa5518fdc54
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Milton, James A.
9e183221-d0d4-4ddb-aeba-0fdde9d31230
Patole, Samson
c35e3cba-1913-4a6a-a78e-942495470f6e
Yin, Huabing
015832c8-0958-47f8-a24c-abc85ae34506
Xiao, Qiang
abe7be8e-6131-403e-aa22-2fa5518fdc54
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Milton, James A., Patole, Samson, Yin, Huabing, Xiao, Qiang, Brown, Tom and Melvin, Tracy (2013) Efficient self-assembly of DNA-functionalized fluorophores and gold nanoparticles with DNA functionalized silicon surfaces: the effect of oligomer spacers. Nucleic Acids Research, 41 (7), e80. (PMID:23361467)

Record type: Article

Abstract

Although strategies for the immobilization of DNA oligonucleotides onto surfaces for bioanalytical and top-down bio-inspired nanobiofabrication approaches are well developed, the effect of introducing spacer molecules between the surface and the DNA oligonucleotide for the hybridization of nanoparticle–DNA conjugates has not been previously assessed in a quantitative manner. The hybridization efficiency of DNA oligonucleotides end-labelled with gold nanoparticles (1.4 or 10 nm diameter) with DNA sequences conjugated to silicon surfaces via hexaethylene glycol phosphate diester oligomer spacers (0, 1, 2, 6 oligomers) was found to be independent of spacer length. To quantify both the density of DNA strands attached to the surfaces and hybridization with the surface-attached DNA, new methodologies have been developed. Firstly, a simple approach based on fluorescence has been developed for determination of the immobilization density of DNA oligonucleotides. Secondly, an approach using mass spectrometry has been created to establish (i) the mean number of DNA oligonucleotides attached to the gold nanoparticles and (ii) the hybridization density of nanoparticle–oligonucleotide conjugates with the silicon surface–attached complementary sequence. These methods and results will be useful for application with nanosensors, the self-assembly of nanoelectronic devices and the attachment of nanoparticles to biomolecules for single-molecule biophysical studies.

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More information

e-pub ahead of print date: 29 January 2013
Published date: April 2013
Organisations: Chemistry, Optoelectronics Research Centre, Ocean and Earth Science, Geochemistry, Electronics & Computer Science

Identifiers

Local EPrints ID: 349390
URI: https://eprints.soton.ac.uk/id/eprint/349390
ISSN: 0305-1048
PURE UUID: a03efa93-e139-403d-833c-148d6fa92948

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Date deposited: 04 Mar 2013 10:18
Last modified: 10 Nov 2017 18:31

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Contributors

Author: James A. Milton
Author: Samson Patole
Author: Huabing Yin
Author: Qiang Xiao
Author: Tom Brown
Author: Tracy Melvin

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