Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion
Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion
Characterization of mammalian NSF (G274E) and Drosophila NSF (comatose) mutants revealed an evolutionarily conserved NSF activity distinct from ATPase-dependent SNARE disassembly that was essential for Golgi membrane fusion. Analysis of mammalian NSF function during cell-free assembly of Golgi cisternae from mitotic Golgi fragments revealed that NSF disassembles Golgi SNAREs during mitotic Golgi fragmentation. A subsequent ATPase-independent NSF activity restricted to the reassembly phase is essential for membrane fusion. NSF/alpha-SNAP catalyze the binding of GATE-16 to GOS-28, a Golgi v-SNARE, in a manner that requires ATP but not ATP hydrolysis. GATE-16 is essential for NSF-driven Golgi reassembly and precludes GOS-28 from binding to its cognate t-SNARE, syntaxin-5. We suggest that this occurs at the inception of Golgi reassembly to protect the v-SNARE and regulate SNARE function.
1161-1173
Müller, Joyce M.M.
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Shorter, James
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Newman, Richard
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Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Sagiv, Yuval
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Elazar, Zvulun
c3c9f4be-1a85-4756-a6b9-d58a0eee12c7
Warren, Graham
b333359b-629a-4a98-8fc4-24566215d10a
Shima, David T.
a84b2872-6c95-46cc-9456-cc3063e02aa8
24 June 2002
Müller, Joyce M.M.
84f4c67c-e699-417f-94cd-f822c1d89295
Shorter, James
cd7c4edb-68d8-472a-8801-0e8a7383a331
Newman, Richard
39f4f482-af9d-4c92-9e28-cf638ca12467
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Sagiv, Yuval
ab8825b5-4d7e-4b0a-9041-025fa9748b6a
Elazar, Zvulun
c3c9f4be-1a85-4756-a6b9-d58a0eee12c7
Warren, Graham
b333359b-629a-4a98-8fc4-24566215d10a
Shima, David T.
a84b2872-6c95-46cc-9456-cc3063e02aa8
Müller, Joyce M.M., Shorter, James, Newman, Richard, Deinhardt, Katrin, Sagiv, Yuval, Elazar, Zvulun, Warren, Graham and Shima, David T.
(2002)
Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion.
PLoS ONE, 157 (7), .
(doi:10.1083/jcb.200202082).
(PMID:12070132)
Abstract
Characterization of mammalian NSF (G274E) and Drosophila NSF (comatose) mutants revealed an evolutionarily conserved NSF activity distinct from ATPase-dependent SNARE disassembly that was essential for Golgi membrane fusion. Analysis of mammalian NSF function during cell-free assembly of Golgi cisternae from mitotic Golgi fragments revealed that NSF disassembles Golgi SNAREs during mitotic Golgi fragmentation. A subsequent ATPase-independent NSF activity restricted to the reassembly phase is essential for membrane fusion. NSF/alpha-SNAP catalyze the binding of GATE-16 to GOS-28, a Golgi v-SNARE, in a manner that requires ATP but not ATP hydrolysis. GATE-16 is essential for NSF-driven Golgi reassembly and precludes GOS-28 from binding to its cognate t-SNARE, syntaxin-5. We suggest that this occurs at the inception of Golgi reassembly to protect the v-SNARE and regulate SNARE function.
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Published date: 24 June 2002
Organisations:
Centre for Biological Sciences
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Local EPrints ID: 349464
URI: http://eprints.soton.ac.uk/id/eprint/349464
ISSN: 1932-6203
PURE UUID: 3a3e3ef5-fd6f-4c72-b674-da6302f4153f
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Date deposited: 05 Mar 2013 14:24
Last modified: 15 Mar 2024 03:45
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Author:
Joyce M.M. Müller
Author:
James Shorter
Author:
Richard Newman
Author:
Yuval Sagiv
Author:
Zvulun Elazar
Author:
Graham Warren
Author:
David T. Shima
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