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The bactericidal effects of dental ultrasound on Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis - an in vitro investigation

The bactericidal effects of dental ultrasound on Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis - an in vitro investigation
The bactericidal effects of dental ultrasound on Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis - an in vitro investigation
This study investigated the possible bactericidal acoustic effects of the dental ultrasonic scaler. Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis suspensions, were subjected to the vibrations of a Cavitron PI insert for 2.5 and 5.0 min in an acoustically-simulated pocket model and the survivors enumerated. The extent of any cavitation occurring within the pocket model to which the statistically significant bactericidal activity observed might be attributed, was determined by 'sonoluminescence', which was then investigated by photomultiplication techniques. However, these failed to detect any sonoluminescence within the pocket space and, moreover, the necessary deflection of the water coolant away from the insert tip, to avoid flooding of the experimental pocket, proved to result in temperatures of 47.6°C and 52.3°C at the respective time intervals, and thereby constituted an alternative possible bactericidal mechanism. Examination of the effects of such temperature changes on the target bacteria then revealed statistically significant differences in the viable counts of both microorganisms after 5.0-min periods, and as such were comparable to those previously detected in relation to the pocket model. Whilst it must be presumed that the bacteriolytic effect observed in the main investigation was due to the incidental temperature changes, in the absence of acoustic cavitation the influence of any associated acoustic microstreaming cannot be discounted. Further investigations to assess the bactericidal potential of acoustic phenomena using a modified experimental to exclude any hyperthermic effects are therefore necessary
1600-051X
432-439
O'Leary, R.
d51b4f10-c098-47df-b4ba-8e9234b23f34
Sved, A.M.
740dba32-cf25-481b-9bba-f0ac8e25e3e3
Davies, E.H.
fcf0b99e-6adc-41c9-bf9d-2c6e2881bdb4
Leighton, T.G.
3e5262ce-1d7d-42eb-b013-fcc5c286bbae
Wilson, M.
eb86ea3a-b4c5-4328-85bf-b20cc96c6c63
Kieser, J.B.
691e19fc-597c-486b-920b-7e58c6220fc6
O'Leary, R.
d51b4f10-c098-47df-b4ba-8e9234b23f34
Sved, A.M.
740dba32-cf25-481b-9bba-f0ac8e25e3e3
Davies, E.H.
fcf0b99e-6adc-41c9-bf9d-2c6e2881bdb4
Leighton, T.G.
3e5262ce-1d7d-42eb-b013-fcc5c286bbae
Wilson, M.
eb86ea3a-b4c5-4328-85bf-b20cc96c6c63
Kieser, J.B.
691e19fc-597c-486b-920b-7e58c6220fc6

O'Leary, R., Sved, A.M., Davies, E.H., Leighton, T.G., Wilson, M. and Kieser, J.B. (1997) The bactericidal effects of dental ultrasound on Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis - an in vitro investigation. Journal of Clinical Periodonology, 24 (6), 432-439. (doi:10.1111/j.1600-051X.1997.tb00208.x). (PMID:9205923)

Record type: Article

Abstract

This study investigated the possible bactericidal acoustic effects of the dental ultrasonic scaler. Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis suspensions, were subjected to the vibrations of a Cavitron PI insert for 2.5 and 5.0 min in an acoustically-simulated pocket model and the survivors enumerated. The extent of any cavitation occurring within the pocket model to which the statistically significant bactericidal activity observed might be attributed, was determined by 'sonoluminescence', which was then investigated by photomultiplication techniques. However, these failed to detect any sonoluminescence within the pocket space and, moreover, the necessary deflection of the water coolant away from the insert tip, to avoid flooding of the experimental pocket, proved to result in temperatures of 47.6°C and 52.3°C at the respective time intervals, and thereby constituted an alternative possible bactericidal mechanism. Examination of the effects of such temperature changes on the target bacteria then revealed statistically significant differences in the viable counts of both microorganisms after 5.0-min periods, and as such were comparable to those previously detected in relation to the pocket model. Whilst it must be presumed that the bacteriolytic effect observed in the main investigation was due to the incidental temperature changes, in the absence of acoustic cavitation the influence of any associated acoustic microstreaming cannot be discounted. Further investigations to assess the bactericidal potential of acoustic phenomena using a modified experimental to exclude any hyperthermic effects are therefore necessary

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Published date: June 1997
Organisations: Acoustics Group

Identifiers

Local EPrints ID: 349537
URI: https://eprints.soton.ac.uk/id/eprint/349537
ISSN: 1600-051X
PURE UUID: 727c423d-d73a-4711-9234-5a3eb45fe614
ORCID for T.G. Leighton: ORCID iD orcid.org/0000-0002-1649-8750

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Date deposited: 07 Mar 2013 11:38
Last modified: 06 Jun 2018 13:08

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