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Can Simpson's paradox explain co-operation in Pseudomonas aeruginosa biofilms?

Penn, Alexandra S, Conibear, Tim C.R., Watson, Richard A., Kraaijeveld, Alex R. and Webb, Jeremy S. (2012) Can Simpson's paradox explain co-operation in Pseudomonas aeruginosa biofilms? FEMS Immunology & Medical Microbiology, 65, (2), pp. 226-235. (doi:10.1111/j.1574-695X.2012.00970.x).

Record type: Article

Abstract

Co-operative behaviours, such as the production of public goods, are commonly displayed by bacteria in biofilms and can enhance their ability to survive in environmental or clinical settings. Non-co-operative cheats commonly arise and should, theoretically, disrupt co-operative behaviour. Its stability therefore requires explanation, but no mechanisms to suppress cheating within biofilms have yet been demonstrated experimentally. Theoretically, repeated aggregation into groups, interleaved with dispersal and remixing, can increase co-operation via a ‘Simpson's paradox’. That is, an increase in the global proportion of co-operators despite a decrease in within-group proportions, via differential growth of groups. We investigate the hypothesis that microcolony formation and dispersal produces a Simpson's paradox that explains bacterial co-operation in biofilms. Using the production of siderophores in Pseudomonas aeruginosa as our model system for co-operation, we use well-documented co-operator and siderophore-deficient cheat strains to measure the frequency of co-operating and cheating individuals, in-situ within-microcolony structures. We detected significant within-type negative density-dependant effects that vary over microcolony development. However, we find no evidence of Simpson's paradox. Instead, we see clear within-microcolony spatial structure (cheats occupying the interior portions of microcolonies) that may violate the assumption required for Simpson's paradox that group members share equally in the public good.

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More information

e-pub ahead of print date: 23 April 2012
Published date: July 2012
Keywords: biofilm development, simpson's paradox, co-pperation, siderophore production, pseudomonas aeruginosa
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 349675
URI: http://eprints.soton.ac.uk/id/eprint/349675
ISSN: 0928-8244
PURE UUID: e7f4362e-c3c0-477a-beee-d1c033fb998c
ORCID for Alex R. Kraaijeveld: ORCID iD orcid.org/0000-0002-8543-2640
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

Catalogue record

Date deposited: 11 Mar 2013 10:23
Last modified: 18 Jul 2017 04:39

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Contributors

Author: Alexandra S Penn
Author: Tim C.R. Conibear
Author: Jeremy S. Webb ORCID iD

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