The University of Southampton
University of Southampton Institutional Repository

Endocytosis and retrograde axonal traffic in motor neurons

Endocytosis and retrograde axonal traffic in motor neurons
Endocytosis and retrograde axonal traffic in motor neurons
Spinal cord motor neurons control voluntary movement by relaying messages that arrive from upper brain centres to the innervated muscles. Despite the importance of motor neurons in human health and disease, the precise control of their membrane dynamics and its effect on motor neuron homoeostasis and survival are poorly understood. In particular, the molecular basis of the co-ordination of specific endocytic events with the axonal retrograde transport pathway is largely unknown. To study these important vesicular trafficking events, we pioneered the use of atoxic fragments of tetanus and botulinum neurotoxins to follow endocytosis and retrograde axonal transport in motor neurons. These neurotoxins bind specifically to pre-synaptic nerve terminals, where they are internalized. Whereas botulinum neurotoxins remain at the neuromuscular junction, tetanus toxin is retrogradely transported along the axon to the cell body, where it is released into the intersynaptic space and is internalized by adjacent inhibitory interneurons. The high neurospecificity and the differential intracellular sorting make tetanus and botulinum neurotoxins ideal tools to study neuronal physiology. In the present review, we discuss recent developments in our understanding of the internalization and trafficking of these molecules in spinal cord motor neurons. Furthermore, we describe the development of a reliable transfection method for motor neurons based on microinjection, which will be extremely useful for dissecting further the molecular basis of membrane dynamics and axonal transport in these cells.
0067-8694
139-150
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Schiavo, Giampietro
0e309b58-ff46-4c10-8eb5-42b380b24223
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Schiavo, Giampietro
0e309b58-ff46-4c10-8eb5-42b380b24223

Deinhardt, Katrin and Schiavo, Giampietro (2005) Endocytosis and retrograde axonal traffic in motor neurons. Biochemical Society Symposium, (72), 139-150. (PMID:15649138)

Record type: Article

Abstract

Spinal cord motor neurons control voluntary movement by relaying messages that arrive from upper brain centres to the innervated muscles. Despite the importance of motor neurons in human health and disease, the precise control of their membrane dynamics and its effect on motor neuron homoeostasis and survival are poorly understood. In particular, the molecular basis of the co-ordination of specific endocytic events with the axonal retrograde transport pathway is largely unknown. To study these important vesicular trafficking events, we pioneered the use of atoxic fragments of tetanus and botulinum neurotoxins to follow endocytosis and retrograde axonal transport in motor neurons. These neurotoxins bind specifically to pre-synaptic nerve terminals, where they are internalized. Whereas botulinum neurotoxins remain at the neuromuscular junction, tetanus toxin is retrogradely transported along the axon to the cell body, where it is released into the intersynaptic space and is internalized by adjacent inhibitory interneurons. The high neurospecificity and the differential intracellular sorting make tetanus and botulinum neurotoxins ideal tools to study neuronal physiology. In the present review, we discuss recent developments in our understanding of the internalization and trafficking of these molecules in spinal cord motor neurons. Furthermore, we describe the development of a reliable transfection method for motor neurons based on microinjection, which will be extremely useful for dissecting further the molecular basis of membrane dynamics and axonal transport in these cells.

Full text not available from this repository.

More information

Published date: 2005
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 349686
URI: http://eprints.soton.ac.uk/id/eprint/349686
ISSN: 0067-8694
PURE UUID: 8cf8a09e-857c-483f-89b5-b357aa540de1
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298

Catalogue record

Date deposited: 11 Mar 2013 16:56
Last modified: 20 Jul 2019 00:40

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×