The University of Southampton
University of Southampton Institutional Repository

Effects of arsenate (As5+) on growth and production of glutathione (GSH) and phytochelatins (PCs) in Chlorella vulgaris

Effects of arsenate (As5+) on growth and production of glutathione (GSH) and phytochelatins (PCs) in Chlorella vulgaris
Effects of arsenate (As5+) on growth and production of glutathione (GSH) and phytochelatins (PCs) in Chlorella vulgaris
The effect of arsenate (As5+) on growth and chlorophyll a production in Chlorella vulgaris, its removal by C. vulgaris and the role of glutathione (GSH) and phytochelatins (PCs) were investigated.

C. vulgaris was tolerant to As5+ at up to 200 mg/L and was capable of consistently removing around 70% of the As5+ present in growth media over a wide range of exposure concentrations. Spectral analysis revealed that PCs and their arsenic-combined complexes were absent indicating that the high bioaccumulation and tolerance to arsenic observed was not due to intracellular chelation. In contrast, GSH was found in all samples ranging from 0.8 mg/L in the control to 6.5mg/L in media containing 200 mg/L As5+ suggesting that GSH plays a more prominent role in the detoxification of As5+ in C. vulgaris than PC. At concentrations below 100 mg/L cell surface binding and other mechanisms may play the primary role in As5+ detoxification, whereas above this concentration As5+ begins to accumulate inside the algal cells and activates a number of intracellular cell defence mechanisms, such as increased production of GSH.

The overall findings complement field studies which suggest C. vulgaris as an increasingly promising low cost As phytoremediation method for developing countries.
1522-6514
834-844
Jiang, Ying
e6e39ee2-e0a6-4e60-9604-ce363c7ce5f9
Jones, Huw
9fcd816f-3f39-4ef5-af4f-3c94fe1e7537
Garelick, Hemda
5c3fbf52-211d-493b-99e0-f41645b9d8dd
Purchase, Diane
1e400105-6374-45b9-8a4a-965f2cde00f4
Jiang, Ying
e6e39ee2-e0a6-4e60-9604-ce363c7ce5f9
Jones, Huw
9fcd816f-3f39-4ef5-af4f-3c94fe1e7537
Garelick, Hemda
5c3fbf52-211d-493b-99e0-f41645b9d8dd
Purchase, Diane
1e400105-6374-45b9-8a4a-965f2cde00f4

Jiang, Ying, Jones, Huw, Garelick, Hemda and Purchase, Diane (2011) Effects of arsenate (As5+) on growth and production of glutathione (GSH) and phytochelatins (PCs) in Chlorella vulgaris. International Journal of Phytoremediation, 13 (8), 834-844. (doi:10.1080/15226514.2010.525560).

Record type: Article

Abstract

The effect of arsenate (As5+) on growth and chlorophyll a production in Chlorella vulgaris, its removal by C. vulgaris and the role of glutathione (GSH) and phytochelatins (PCs) were investigated.

C. vulgaris was tolerant to As5+ at up to 200 mg/L and was capable of consistently removing around 70% of the As5+ present in growth media over a wide range of exposure concentrations. Spectral analysis revealed that PCs and their arsenic-combined complexes were absent indicating that the high bioaccumulation and tolerance to arsenic observed was not due to intracellular chelation. In contrast, GSH was found in all samples ranging from 0.8 mg/L in the control to 6.5mg/L in media containing 200 mg/L As5+ suggesting that GSH plays a more prominent role in the detoxification of As5+ in C. vulgaris than PC. At concentrations below 100 mg/L cell surface binding and other mechanisms may play the primary role in As5+ detoxification, whereas above this concentration As5+ begins to accumulate inside the algal cells and activates a number of intracellular cell defence mechanisms, such as increased production of GSH.

The overall findings complement field studies which suggest C. vulgaris as an increasingly promising low cost As phytoremediation method for developing countries.

Text
As paper scholar text.pdf - Accepted Manuscript
Download (497kB)

More information

e-pub ahead of print date: 26 May 2011
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 349729
URI: http://eprints.soton.ac.uk/id/eprint/349729
ISSN: 1522-6514
PURE UUID: 7dbc7a96-dc61-402f-abf0-a6f64a12acda

Catalogue record

Date deposited: 11 Mar 2013 12:42
Last modified: 28 Oct 2019 21:10

Export record

Altmetrics

Contributors

Author: Ying Jiang
Author: Huw Jones
Author: Hemda Garelick
Author: Diane Purchase

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×