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The local matrix distribution and the functional development of tissue engineered cartilage, a finite element study

The local matrix distribution and the functional development of tissue engineered cartilage, a finite element study
The local matrix distribution and the functional development of tissue engineered cartilage, a finite element study
Assessment of the functionality of tissue engineered cartilage constructs is hampered by the lack of correlation between global measurements of extra cellular matrix constituents and the global mechanical properties. Based on patterns of matrix deposition around individual cells, it has been hypothesized previously, that mechanical functionality arises when contact occurs between zones of matrix associated with individual cells. The objective of this study is to determine whether the local distribution of newly synthesized extracellular matrix components contributes to the evolution of the mechanical properties of tissue engineered cartilage constructs. A computational homogenization approach was adopted, based on the concept of a periodic representative volume element. Local transport and immobilization of newly synthesized matrix components were described. Mechanical properties were taken dependent on the local matrix concentration and subsequently the global aggregate modulus and hydraulic permeability were derived. The transport parameters were varied to assess the effect of the evolving matrix distribution during culture. The results indicate that the overall stiffness and permeability are to a large extent insensitive to differences in local matrix distribution. This emphasizes the need for caution in the visual interpretation of tissue functionality from histology and underlines the importance of complementary measurements of the matrix's intrinsic molecular organization.
0090-6964
1718-1727
Sengers, B.G.
d6b771b1-4ede-48c5-9644-fa86503941aa
Van Donkelaar, C.C.
fa75933d-bf74-4366-95d0-3694b6cd4fbf
Oomens, C.W.J.
a8310c52-8ab4-4652-b2d6-82269a3c7438
Baaijens, F.P.T.
5d9e63e2-7d7b-4f64-b942-29121121dce0
Sengers, B.G.
d6b771b1-4ede-48c5-9644-fa86503941aa
Van Donkelaar, C.C.
fa75933d-bf74-4366-95d0-3694b6cd4fbf
Oomens, C.W.J.
a8310c52-8ab4-4652-b2d6-82269a3c7438
Baaijens, F.P.T.
5d9e63e2-7d7b-4f64-b942-29121121dce0

Sengers, B.G., Van Donkelaar, C.C., Oomens, C.W.J. and Baaijens, F.P.T. (2004) The local matrix distribution and the functional development of tissue engineered cartilage, a finite element study. Annals of Biomedical Engineering, 32 (12), 1718-1727. (doi:10.1007/s10439-004-7824-3). (PMID:15675683)

Record type: Article

Abstract

Assessment of the functionality of tissue engineered cartilage constructs is hampered by the lack of correlation between global measurements of extra cellular matrix constituents and the global mechanical properties. Based on patterns of matrix deposition around individual cells, it has been hypothesized previously, that mechanical functionality arises when contact occurs between zones of matrix associated with individual cells. The objective of this study is to determine whether the local distribution of newly synthesized extracellular matrix components contributes to the evolution of the mechanical properties of tissue engineered cartilage constructs. A computational homogenization approach was adopted, based on the concept of a periodic representative volume element. Local transport and immobilization of newly synthesized matrix components were described. Mechanical properties were taken dependent on the local matrix concentration and subsequently the global aggregate modulus and hydraulic permeability were derived. The transport parameters were varied to assess the effect of the evolving matrix distribution during culture. The results indicate that the overall stiffness and permeability are to a large extent insensitive to differences in local matrix distribution. This emphasizes the need for caution in the visual interpretation of tissue functionality from histology and underlines the importance of complementary measurements of the matrix's intrinsic molecular organization.

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Published date: December 2004
Organisations: Faculty of Engineering and the Environment

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Local EPrints ID: 349743
URI: http://eprints.soton.ac.uk/id/eprint/349743
ISSN: 0090-6964
PURE UUID: bb851e33-7e4a-4041-8570-771cfdc3ba69
ORCID for B.G. Sengers: ORCID iD orcid.org/0000-0001-5859-6984

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Date deposited: 12 Mar 2013 14:26
Last modified: 26 Nov 2019 01:44

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Contributors

Author: B.G. Sengers ORCID iD
Author: C.C. Van Donkelaar
Author: C.W.J. Oomens
Author: F.P.T. Baaijens

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