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Computational study of culture conditions and nutrient supply in cartilage tissue engineering

Computational study of culture conditions and nutrient supply in cartilage tissue engineering
Computational study of culture conditions and nutrient supply in cartilage tissue engineering
Different culture conditions for cartilage tissue engineering were evaluated with respect to the supply of oxygen and glucose and the accumulation of lactate. A computational approach was adopted in which the culture configurations were modeled as a batch process and transport was considered within constructs seeded at high cell concentrations and of clinically relevant dimensions. To assess the extent to which mass transfer can be influenced theoretically, extreme cases were distinguished in which the culture medium surrounding the construct was assumed either completely static or well mixed and fully oxygenated. It can be concluded that severe oxygen depletion and lactate accumulation can occur within constructs for cartilage tissue engineering. However, the results also indicate that transport restrictions are not insurmountable, providing that the medium is well homogenized and oxygenated and the construct's surfaces are sufficiently exposed to the medium. The large variation in uptake rates of chondrocytes indicates that for any specific application the quantification of cellular utilization rates, depending on the cell source and culture conditions, is an essential starting point for optimizing culture protocols.
1252-1261
Sengers, B.G.
d6b771b1-4ede-48c5-9644-fa86503941aa
van Donkelaar, C.C.
fa75933d-bf74-4366-95d0-3694b6cd4fbf
Oomens, C.W.J.
a8310c52-8ab4-4652-b2d6-82269a3c7438
Baaijens, F.P.T.
5d9e63e2-7d7b-4f64-b942-29121121dce0
Sengers, B.G.
d6b771b1-4ede-48c5-9644-fa86503941aa
van Donkelaar, C.C.
fa75933d-bf74-4366-95d0-3694b6cd4fbf
Oomens, C.W.J.
a8310c52-8ab4-4652-b2d6-82269a3c7438
Baaijens, F.P.T.
5d9e63e2-7d7b-4f64-b942-29121121dce0

Sengers, B.G., van Donkelaar, C.C., Oomens, C.W.J. and Baaijens, F.P.T. (2005) Computational study of culture conditions and nutrient supply in cartilage tissue engineering. Biotechnology Progress, 21 (4), 1252-1261. (doi:10.1021/bp0500157). (PMID:16080709)

Record type: Article

Abstract

Different culture conditions for cartilage tissue engineering were evaluated with respect to the supply of oxygen and glucose and the accumulation of lactate. A computational approach was adopted in which the culture configurations were modeled as a batch process and transport was considered within constructs seeded at high cell concentrations and of clinically relevant dimensions. To assess the extent to which mass transfer can be influenced theoretically, extreme cases were distinguished in which the culture medium surrounding the construct was assumed either completely static or well mixed and fully oxygenated. It can be concluded that severe oxygen depletion and lactate accumulation can occur within constructs for cartilage tissue engineering. However, the results also indicate that transport restrictions are not insurmountable, providing that the medium is well homogenized and oxygenated and the construct's surfaces are sufficiently exposed to the medium. The large variation in uptake rates of chondrocytes indicates that for any specific application the quantification of cellular utilization rates, depending on the cell source and culture conditions, is an essential starting point for optimizing culture protocols.

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More information

Published date: July 2005
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 349744
URI: http://eprints.soton.ac.uk/id/eprint/349744
PURE UUID: 4f525d2a-8826-44b8-b5f5-ef5ae61b4a64
ORCID for B.G. Sengers: ORCID iD orcid.org/0000-0001-5859-6984

Catalogue record

Date deposited: 12 Mar 2013 14:42
Last modified: 19 Nov 2019 01:45

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Contributors

Author: B.G. Sengers ORCID iD
Author: C.C. van Donkelaar
Author: C.W.J. Oomens
Author: F.P.T. Baaijens

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