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Nutrient utilization by bovine articular chondrocytes: a combined experimental and theoretical approach

Nutrient utilization by bovine articular chondrocytes: a combined experimental and theoretical approach
Nutrient utilization by bovine articular chondrocytes: a combined experimental and theoretical approach
A combined experimental-numerical approach was adopted to characterize glucose and oxygen uptake and lactate production by bovine articular chondrocytes in a model system. For a wide range of cell concentrations, cells in agarose were supplemented with either low or high glucose medium. During an initial culture phase of 48 h, oxygen was monitored noninvasively using a biosensor system. Glucose and lactate were determined by medium sampling. In order to quantify glucose and oxygen uptake, a finite element approach was adopted to describe diffusion and uptake in the experimental model. Numerical predictions of lactate, based on simple relations for cell metabolism, were found to agree well for low glucose, but not for high glucose medium. Oxygen did not play a role in either case. Given the close association between chondrocyte energy metabolism and matrix synthesis, a quantifiable prediction of utilization can present a valuable contribution in the optimization of tissue engineering conditions.
0148-0731
758-766
Sengers, Bram G.
d6b771b1-4ede-48c5-9644-fa86503941aa
Heywood, Hannah K.
7cc8565c-d7a8-496a-ab70-f53f4659bf6d
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
Oomens, Cees W.J.
e8a85b85-3719-4909-9f82-4f03d8a43263
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Sengers, Bram G.
d6b771b1-4ede-48c5-9644-fa86503941aa
Heywood, Hannah K.
7cc8565c-d7a8-496a-ab70-f53f4659bf6d
Lee, David A.
1c62bb7c-fe96-442d-b518-13dd6d558871
Oomens, Cees W.J.
e8a85b85-3719-4909-9f82-4f03d8a43263
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf

Sengers, Bram G., Heywood, Hannah K., Lee, David A., Oomens, Cees W.J. and Bader, Dan L. (2005) Nutrient utilization by bovine articular chondrocytes: a combined experimental and theoretical approach. Journal of Biomechanical Engineering, 127 (5), 758-766. (doi:10.1115/1.1993664). (PMID:16248305)

Record type: Article

Abstract

A combined experimental-numerical approach was adopted to characterize glucose and oxygen uptake and lactate production by bovine articular chondrocytes in a model system. For a wide range of cell concentrations, cells in agarose were supplemented with either low or high glucose medium. During an initial culture phase of 48 h, oxygen was monitored noninvasively using a biosensor system. Glucose and lactate were determined by medium sampling. In order to quantify glucose and oxygen uptake, a finite element approach was adopted to describe diffusion and uptake in the experimental model. Numerical predictions of lactate, based on simple relations for cell metabolism, were found to agree well for low glucose, but not for high glucose medium. Oxygen did not play a role in either case. Given the close association between chondrocyte energy metabolism and matrix synthesis, a quantifiable prediction of utilization can present a valuable contribution in the optimization of tissue engineering conditions.

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Published date: October 2005
Organisations: Faculty of Engineering and the Environment

Identifiers

Local EPrints ID: 349745
URI: http://eprints.soton.ac.uk/id/eprint/349745
ISSN: 0148-0731
PURE UUID: 4bac3cde-30b7-4aab-8bd1-80b9afcf67bb
ORCID for Bram G. Sengers: ORCID iD orcid.org/0000-0001-5859-6984
ORCID for Dan L. Bader: ORCID iD orcid.org/0000-0002-1208-3507

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Date deposited: 12 Mar 2013 14:37
Last modified: 03 Dec 2019 01:46

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Contributors

Author: Bram G. Sengers ORCID iD
Author: Hannah K. Heywood
Author: David A. Lee
Author: Cees W.J. Oomens
Author: Dan L. Bader ORCID iD

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