The effect of base-pair sequence on electrochemically driven denaturation
The effect of base-pair sequence on electrochemically driven denaturation
Application of a voltage ramp can result in denaturation of dsDNA and strand separation. We show that the potential at which half of the surface immobilised duplexes denature (the melting potential, Em) directly correlates with the calculated nearest neighbour and experimental melting temperatures; Tm, for a duplex is solution. The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.
dsDNA denaturation, surface enhanced raman spectroscopy, electrochemical melting, nearest neighbour model
7-13
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Gao, Rachel
47cbe2e0-7030-4348-a6df-35673fa2a253
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
June 2012
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Gao, Rachel
47cbe2e0-7030-4348-a6df-35673fa2a253
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Johnson, Robert P., Gao, Rachel, Brown, Tom and Bartlett, Philip N.
(2012)
The effect of base-pair sequence on electrochemically driven denaturation.
Bioelectrochemistry, 85, .
(doi:10.1016/j.bioelechem.2011.11.003).
Abstract
Application of a voltage ramp can result in denaturation of dsDNA and strand separation. We show that the potential at which half of the surface immobilised duplexes denature (the melting potential, Em) directly correlates with the calculated nearest neighbour and experimental melting temperatures; Tm, for a duplex is solution. The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.
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Published date: June 2012
Keywords:
dsDNA denaturation, surface enhanced raman spectroscopy, electrochemical melting, nearest neighbour model
Organisations:
Chemistry
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Local EPrints ID: 349750
URI: http://eprints.soton.ac.uk/id/eprint/349750
PURE UUID: bdff78f0-18cc-4e43-9652-8353b3ee8568
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Date deposited: 08 Mar 2013 17:59
Last modified: 15 Mar 2024 02:44
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Author:
Robert P. Johnson
Author:
Rachel Gao
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