A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations
A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations
A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and ?F508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule.
14099-14107
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Richardson, James A.
51db9f73-a136-48af-8722-21d46906cf40
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
2012
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Richardson, James A.
51db9f73-a136-48af-8722-21d46906cf40
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Johnson, Robert P., Richardson, James A., Brown, Tom and Bartlett, Philip N.
(2012)
A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations.
Journal of the American Chemical Society, 134 (34), .
(doi:10.1021/ja304663t).
Abstract
A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and ?F508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule.
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e-pub ahead of print date: 26 July 2012
Published date: 2012
Organisations:
Chemistry
Identifiers
Local EPrints ID: 349756
URI: http://eprints.soton.ac.uk/id/eprint/349756
ISSN: 0002-7863
PURE UUID: e70787e6-9f61-4671-be28-5c72e881fecf
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Date deposited: 08 Mar 2013 17:40
Last modified: 15 Mar 2024 02:44
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Author:
Robert P. Johnson
Author:
James A. Richardson
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