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A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations

A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations
A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations
A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and ?F508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule.
0002-7863
14099-14107
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Richardson, James A.
51db9f73-a136-48af-8722-21d46906cf40
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075
Johnson, Robert P.
5dabdb76-784a-4d20-bed7-447f2a42705b
Richardson, James A.
51db9f73-a136-48af-8722-21d46906cf40
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Bartlett, Philip N.
d99446db-a59d-4f89-96eb-f64b5d8bb075

Johnson, Robert P., Richardson, James A., Brown, Tom and Bartlett, Philip N. (2012) A label-free, electrochemical SERS-based assay for detection of DNA hybridization and discrimination of mutations. Journal of the American Chemical Society, 134 (34), 14099-14107. (doi:10.1021/ja304663t).

Record type: Article

Abstract

A label-free, surface-enhanced Raman spectroscopy-based assay for detecting DNA hybridization at an electrode surface and for distinguishing between mutations in DNA is demonstrated. Surface-immobilized DNA is exposed to a binding agent that is selective for dsDNA and acts as a reporter molecule. Upon application of a negative potential, the dsDNA denatures into its constituent strands, and the changes in the spectra of the reporter molecule are monitored. This method has been used to distinguish between a wild-type, 1653C/T single-point mutation and ?F508 triplet deletion in the CFTR gene. The use of dsDNA-selective binding agents as reporter molecules in a discrimination assay removes the burden of synthetically modifying the target to be detected, while retaining flexibility in the choice of the reporter molecule.

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e-pub ahead of print date: 26 July 2012
Published date: 2012
Organisations: Chemistry

Identifiers

Local EPrints ID: 349756
URI: http://eprints.soton.ac.uk/id/eprint/349756
ISSN: 0002-7863
PURE UUID: e70787e6-9f61-4671-be28-5c72e881fecf
ORCID for Philip N. Bartlett: ORCID iD orcid.org/0000-0002-7300-6900

Catalogue record

Date deposited: 08 Mar 2013 17:40
Last modified: 19 Nov 2019 02:00

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