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Alternative complex formation of the Ca2+-regulated protein kinase CIPK1 controls abscisic acid-dependent and independent stress responses in Arabidopsis

Alternative complex formation of the Ca2+-regulated protein kinase CIPK1 controls abscisic acid-dependent and independent stress responses in Arabidopsis
Alternative complex formation of the Ca2+-regulated protein kinase CIPK1 controls abscisic acid-dependent and independent stress responses in Arabidopsis
Intracellular release of calcium ions belongs to the earliest events in cellular stress perception. The molecular mechanisms integrating signals from different environmental cues and translating them into an optimized response are largely unknown. We report here the functional characterization of CIPK1, a protein kinase interacting strongly with the calcium sensors CBL1 and CBL9. Comparison of the expression patterns indicates that the three proteins execute their functions in the same tissues. Physical interaction of CIPK1 with CBL1 and CBL9 targets the kinase to the plasma membrane. We show that, similarly to loss of CBL9 function, mutation of either CBL1 or CIPK1 renders plants hypersensitive to osmotic stress. Remarkably, in contrast to the cbl1 mutant and similarly to the cbl9 mutant, loss of CIPK1 function impairs abscisic acid (ABA) responsiveness. We therefore suggest that, by alternative complex formation with either CBL1 or CBL9, the kinase CIPK1 represents a convergence point for ABA-dependent and ABA-independent stress responses. Based on our genetic, physiological and protein-protein interaction data, we propose a general model for information processing in calcium-regulated signalling networks.
calcium signalling, aba, stress response, protein kinase, cbl, cipk
0960-7412
857-872
D'Angelo, Cecilia
0d35b03b-684d-43aa-a57a-87212ab07ee1
Weinl, Stefan
2f78f0c7-3be9-4b12-8ddf-364731ab5071
Batistic, Oliver
e36a3a07-28aa-4549-87f9-88a791fdf1eb
Pandey, Girdhar K.
cecd6b35-fc04-443e-a276-f5b4e6997507
Cheong, Yong Hwa
1d213a41-44ca-451b-8c2a-d33e73080e3d
Schültke, Stefanie
a14e3ffe-7a20-4bdf-8e12-abf9924f2e49
Albrecht, Veronica
b40d66fc-ce9f-4caa-96c9-59ad07b3a64e
Ehlert, Britta
9a1948e0-feeb-4fb7-bfbf-a628df89be0a
Schulz, Burkhard
aff757fe-0766-49ad-b25a-d446b9dd8a6a
Harter, Klaus
c05227a4-f56e-4e9c-8b2a-571130c1ecea
Luan, Sheng
baa8b704-cf99-4c11-8eac-f754b4b83223
Bock, Ralph
ef49a695-9708-4ad3-9221-2a100b630f99
Kudla, Jörg
662352ce-945f-459d-9572-e8875d39b2f3
D'Angelo, Cecilia
0d35b03b-684d-43aa-a57a-87212ab07ee1
Weinl, Stefan
2f78f0c7-3be9-4b12-8ddf-364731ab5071
Batistic, Oliver
e36a3a07-28aa-4549-87f9-88a791fdf1eb
Pandey, Girdhar K.
cecd6b35-fc04-443e-a276-f5b4e6997507
Cheong, Yong Hwa
1d213a41-44ca-451b-8c2a-d33e73080e3d
Schültke, Stefanie
a14e3ffe-7a20-4bdf-8e12-abf9924f2e49
Albrecht, Veronica
b40d66fc-ce9f-4caa-96c9-59ad07b3a64e
Ehlert, Britta
9a1948e0-feeb-4fb7-bfbf-a628df89be0a
Schulz, Burkhard
aff757fe-0766-49ad-b25a-d446b9dd8a6a
Harter, Klaus
c05227a4-f56e-4e9c-8b2a-571130c1ecea
Luan, Sheng
baa8b704-cf99-4c11-8eac-f754b4b83223
Bock, Ralph
ef49a695-9708-4ad3-9221-2a100b630f99
Kudla, Jörg
662352ce-945f-459d-9572-e8875d39b2f3

D'Angelo, Cecilia, Weinl, Stefan, Batistic, Oliver, Pandey, Girdhar K., Cheong, Yong Hwa, Schültke, Stefanie, Albrecht, Veronica, Ehlert, Britta, Schulz, Burkhard, Harter, Klaus, Luan, Sheng, Bock, Ralph and Kudla, Jörg (2006) Alternative complex formation of the Ca2+-regulated protein kinase CIPK1 controls abscisic acid-dependent and independent stress responses in Arabidopsis. The Plant Journal, 48 (6), 857-872. (doi:10.1111/j.1365-313X.2006.02921.x). (PMID:17092313)

Record type: Article

Abstract

Intracellular release of calcium ions belongs to the earliest events in cellular stress perception. The molecular mechanisms integrating signals from different environmental cues and translating them into an optimized response are largely unknown. We report here the functional characterization of CIPK1, a protein kinase interacting strongly with the calcium sensors CBL1 and CBL9. Comparison of the expression patterns indicates that the three proteins execute their functions in the same tissues. Physical interaction of CIPK1 with CBL1 and CBL9 targets the kinase to the plasma membrane. We show that, similarly to loss of CBL9 function, mutation of either CBL1 or CIPK1 renders plants hypersensitive to osmotic stress. Remarkably, in contrast to the cbl1 mutant and similarly to the cbl9 mutant, loss of CIPK1 function impairs abscisic acid (ABA) responsiveness. We therefore suggest that, by alternative complex formation with either CBL1 or CBL9, the kinase CIPK1 represents a convergence point for ABA-dependent and ABA-independent stress responses. Based on our genetic, physiological and protein-protein interaction data, we propose a general model for information processing in calcium-regulated signalling networks.

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e-pub ahead of print date: 6 November 2006
Published date: December 2006
Keywords: calcium signalling, aba, stress response, protein kinase, cbl, cipk
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 349900
URI: http://eprints.soton.ac.uk/id/eprint/349900
ISSN: 0960-7412
PURE UUID: 9e6ca6c1-dc47-4560-85ab-55c01080fe3c

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Date deposited: 13 Mar 2013 12:05
Last modified: 16 Jul 2019 21:41

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Contributors

Author: Stefan Weinl
Author: Oliver Batistic
Author: Girdhar K. Pandey
Author: Yong Hwa Cheong
Author: Stefanie Schültke
Author: Veronica Albrecht
Author: Britta Ehlert
Author: Burkhard Schulz
Author: Klaus Harter
Author: Sheng Luan
Author: Ralph Bock
Author: Jörg Kudla

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