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Autotrophic ammonia oxidation in a deep-sea hydrothermal plume

Autotrophic ammonia oxidation in a deep-sea hydrothermal plume
Autotrophic ammonia oxidation in a deep-sea hydrothermal plume
Direct evidence for autotrophic ammonia oxidation is documented for the first time in a deep-sea hydrothermal plume. Elevated NH(4) (+) concentrations of up to 341+/-136 nM were recorded in the plume core at Main Endeavour Field, Juan de Fuca Ridge. This fueled autotrophic ammonia oxidation rates as high as 91 nM day(-1), or 92% of the total net NH(4) (+) removal. High abundance of ammonia-oxidizing bacteria was detected using fluorescence in situ hybridization. Ammonia-oxidizing bacteria within the plume core (1.0-1.4x10(4) cells ml(-1)) accounted for 7.0-7.5% of the total microbial community, and were at least as abundant as methanotrophs. Ammonia-oxidizing bacteria were a substantial component of the particle-associated communities (up to 51%), with a predominance of the r-strategist Nitrosomonas-like cells. In situ chemolithoautotrophic organic carbon production via ammonia oxidation may yield 3.9-18 mg C m(-2) day(-1) within the plume directly over Main Endeavour Field. This rate was comparable to that determined for methane oxidation in a previous study, or at least four-fold greater than the flux of photosynthetic carbon reaching plume depths measured in another study. Hence, autotrophic ammonia oxidation in the neutrally buoyant hydrothermal plume is significant to both carbon and nitrogen cycling in the deep-sea water column at Endeavour, and represents another important link between seafloor hydrothermal systems and deep-sea biogeochemistry.
ammonia oxidation, chemolithoautotrophy, in situ organic carbon production, hydrothermal plume, fluorescence in situ hybridization, endeavour, mid-ocean ridge, nitrification, marine nitrogen cycling
191-206
Lam, Phyllis
996aef80-a15d-4827-aed8-1b97b378f6ad
Cowen, James P.
5986604a-fab2-4269-8bcc-1876ba9cf15f
Jones, Ronald D.
c78b3d85-1a07-479b-a1af-236aab629ae6
Lam, Phyllis
996aef80-a15d-4827-aed8-1b97b378f6ad
Cowen, James P.
5986604a-fab2-4269-8bcc-1876ba9cf15f
Jones, Ronald D.
c78b3d85-1a07-479b-a1af-236aab629ae6

Lam, Phyllis, Cowen, James P. and Jones, Ronald D. (2004) Autotrophic ammonia oxidation in a deep-sea hydrothermal plume. FEMS Microbiology Ecology, 47 (2), 191-206. (doi:10.1016/S0168-6496(03)00256-3). (PMID:19712334)

Record type: Article

Abstract

Direct evidence for autotrophic ammonia oxidation is documented for the first time in a deep-sea hydrothermal plume. Elevated NH(4) (+) concentrations of up to 341+/-136 nM were recorded in the plume core at Main Endeavour Field, Juan de Fuca Ridge. This fueled autotrophic ammonia oxidation rates as high as 91 nM day(-1), or 92% of the total net NH(4) (+) removal. High abundance of ammonia-oxidizing bacteria was detected using fluorescence in situ hybridization. Ammonia-oxidizing bacteria within the plume core (1.0-1.4x10(4) cells ml(-1)) accounted for 7.0-7.5% of the total microbial community, and were at least as abundant as methanotrophs. Ammonia-oxidizing bacteria were a substantial component of the particle-associated communities (up to 51%), with a predominance of the r-strategist Nitrosomonas-like cells. In situ chemolithoautotrophic organic carbon production via ammonia oxidation may yield 3.9-18 mg C m(-2) day(-1) within the plume directly over Main Endeavour Field. This rate was comparable to that determined for methane oxidation in a previous study, or at least four-fold greater than the flux of photosynthetic carbon reaching plume depths measured in another study. Hence, autotrophic ammonia oxidation in the neutrally buoyant hydrothermal plume is significant to both carbon and nitrogen cycling in the deep-sea water column at Endeavour, and represents another important link between seafloor hydrothermal systems and deep-sea biogeochemistry.

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More information

Published date: 15 February 2004
Keywords: ammonia oxidation, chemolithoautotrophy, in situ organic carbon production, hydrothermal plume, fluorescence in situ hybridization, endeavour, mid-ocean ridge, nitrification, marine nitrogen cycling
Organisations: Ocean Biochemistry & Ecosystems

Identifiers

Local EPrints ID: 350166
URI: http://eprints.soton.ac.uk/id/eprint/350166
PURE UUID: 35b4ff12-ea7d-4592-b950-3b5e7f55daff
ORCID for Phyllis Lam: ORCID iD orcid.org/0000-0003-2067-171X

Catalogue record

Date deposited: 19 Mar 2013 09:50
Last modified: 18 Feb 2021 17:22

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