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16S rRNA gene-based molecular analysis of mat-forming and accompanying bacteria covering organically-enriched marine sediments underlying a salmon farm in Southern Chile (Calbuco Island)

16S rRNA gene-based molecular analysis of mat-forming and accompanying bacteria covering organically-enriched marine sediments underlying a salmon farm in Southern Chile (Calbuco Island)
16S rRNA gene-based molecular analysis of mat-forming and accompanying bacteria covering organically-enriched marine sediments underlying a salmon farm in Southern Chile (Calbuco Island)
The mat forming bacteria covering organic matter-enriched and anoxic marine sediments underlying a salmon farm in Southern Chile, were examined using 16S rRNA gene phylogenies. This mat was absent in the sea bed outside the direct influence of the farm (360 m outside fish cages). Based on nearly complete 16S rRNA gene sequences (~1500 bp), matforming filamentous cells were settled as the sulphur-oxidizing and putatively dissimilative nitrate reducing Beggiatoa spp., being closely related (up to 97% sequence identity) to Beggiatoa spp. identified in eutrophic shallow sediments in northern Europe (Danish Limfjorden and German Dangast inlets). Their phylogenetic affiliation was consistent with their morphology as vacuolated and sulphur-containing cells arranged on tandem along trichomes (18 to 28 ?m diameter). Additionally, deltaproteobacterial sulphate reducers, Sulfurospirillum, Sulfurovum and Fusibacter were detected according to partial 16S rRNA gene sequences (~500 bp). Their concurrence with Beggiatoa suggested an intense and complex sulphur cycle within the surface of these aquaculture affected sediments, which may have important implications for the necessity of more efficient benthic bioremediation of finfish aquaculture in Chile and worldwide.
16S rRNA genes, beggiatoa mat, salmon farming sediments, sulphur cycle
0717-652X
125-135
Aranda, Carlos
14792282-f2e8-4a17-849e-a55f94a6f6d5
Paredes, Javier
9277eb48-d0a0-4100-a12a-83b25aa7bc44
Valenzuela, Cristian
e48bee35-f4ab-4bf4-bbd4-a38699898996
Lam, Phyllis
996aef80-a15d-4827-aed8-1b97b378f6ad
Guillou, Laure
4c1cc072-9123-4a4a-ac9b-87e66eefbe66
Aranda, Carlos
14792282-f2e8-4a17-849e-a55f94a6f6d5
Paredes, Javier
9277eb48-d0a0-4100-a12a-83b25aa7bc44
Valenzuela, Cristian
e48bee35-f4ab-4bf4-bbd4-a38699898996
Lam, Phyllis
996aef80-a15d-4827-aed8-1b97b378f6ad
Guillou, Laure
4c1cc072-9123-4a4a-ac9b-87e66eefbe66

Aranda, Carlos, Paredes, Javier, Valenzuela, Cristian, Lam, Phyllis and Guillou, Laure (2010) 16S rRNA gene-based molecular analysis of mat-forming and accompanying bacteria covering organically-enriched marine sediments underlying a salmon farm in Southern Chile (Calbuco Island). Gayana, 74 (2), 125-135. (doi:10.4067/S0717-65382010000200006).

Record type: Article

Abstract

The mat forming bacteria covering organic matter-enriched and anoxic marine sediments underlying a salmon farm in Southern Chile, were examined using 16S rRNA gene phylogenies. This mat was absent in the sea bed outside the direct influence of the farm (360 m outside fish cages). Based on nearly complete 16S rRNA gene sequences (~1500 bp), matforming filamentous cells were settled as the sulphur-oxidizing and putatively dissimilative nitrate reducing Beggiatoa spp., being closely related (up to 97% sequence identity) to Beggiatoa spp. identified in eutrophic shallow sediments in northern Europe (Danish Limfjorden and German Dangast inlets). Their phylogenetic affiliation was consistent with their morphology as vacuolated and sulphur-containing cells arranged on tandem along trichomes (18 to 28 ?m diameter). Additionally, deltaproteobacterial sulphate reducers, Sulfurospirillum, Sulfurovum and Fusibacter were detected according to partial 16S rRNA gene sequences (~500 bp). Their concurrence with Beggiatoa suggested an intense and complex sulphur cycle within the surface of these aquaculture affected sediments, which may have important implications for the necessity of more efficient benthic bioremediation of finfish aquaculture in Chile and worldwide.

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Published date: 2010
Keywords: 16S rRNA genes, beggiatoa mat, salmon farming sediments, sulphur cycle
Organisations: Ocean Biochemistry & Ecosystems

Identifiers

Local EPrints ID: 350170
URI: https://eprints.soton.ac.uk/id/eprint/350170
ISSN: 0717-652X
PURE UUID: 6d6880b2-b8df-4dce-8f25-096072155870
ORCID for Phyllis Lam: ORCID iD orcid.org/0000-0003-2067-171X

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Date deposited: 19 Mar 2013 10:24
Last modified: 07 Aug 2019 00:33

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