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Photophysiological and photosynthetic complex changes during iron starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942

Photophysiological and photosynthetic complex changes during iron starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942
Photophysiological and photosynthetic complex changes during iron starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942
Iron is an essential component in many protein complexes involved in photosynthesis, but environmental iron availability is often low as oxidized forms of iron are insoluble in water. To adjust to low environmental iron levels, cyanobacteria undergo numerous changes to balance their iron budget and mitigate the physiological effects of iron depletion. We investigated changes in key protein abundances and photophysiological parameters in the model cyanobacteria Synechococcus PCC 7942 and Synechocystis PCC 6803 over a 120 hour time course of iron deprivation. The iron stress induced protein (IsiA) accumulated to high levels within 48 h of the onset of iron deprivation, reaching a molar ratio of ~42 IsiA : Photosystem I in Synechococcus PCC 7942 and ~12 IsiA : Photosystem I in Synechocystis PCC 6803. Concomitantly the iron-rich complexes Cytochrome b6f and Photosystem I declined in abundance, leading to a decrease in the Photosystem I : Photosystem II ratio. Chlorophyll fluorescence analyses showed a drop in electron transport per Photosystem II in Synechococcus, but not in Synechocystis after iron depletion. We found no evidence that the accumulated IsiA contributes to light capture by Photosystem II complexes.
1932-6203
e59861
Fraser, J.M.
d3cdabab-85fc-4e5d-980a-a6eb7fe57aea
Tulk, S.E.
2c11c148-c9e9-4795-a822-87d3317511fc
Campbell, D.A.
ba2c92ad-c43f-47a1-9482-76dc928d3984
Bibby, T.S.
e04ea079-dd90-4ead-9840-00882de27ebd
Cockshutt, A.M.
89d2d81d-50d1-447b-9bae-10cffd9c18b5
Fraser, J.M.
d3cdabab-85fc-4e5d-980a-a6eb7fe57aea
Tulk, S.E.
2c11c148-c9e9-4795-a822-87d3317511fc
Campbell, D.A.
ba2c92ad-c43f-47a1-9482-76dc928d3984
Bibby, T.S.
e04ea079-dd90-4ead-9840-00882de27ebd
Cockshutt, A.M.
89d2d81d-50d1-447b-9bae-10cffd9c18b5

Fraser, J.M., Tulk, S.E., Campbell, D.A., Bibby, T.S. and Cockshutt, A.M. (2013) Photophysiological and photosynthetic complex changes during iron starvation in Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942. PLoS ONE, 8 (3), e59861. (doi:10.1371/journal.pone.0059861).

Record type: Article

Abstract

Iron is an essential component in many protein complexes involved in photosynthesis, but environmental iron availability is often low as oxidized forms of iron are insoluble in water. To adjust to low environmental iron levels, cyanobacteria undergo numerous changes to balance their iron budget and mitigate the physiological effects of iron depletion. We investigated changes in key protein abundances and photophysiological parameters in the model cyanobacteria Synechococcus PCC 7942 and Synechocystis PCC 6803 over a 120 hour time course of iron deprivation. The iron stress induced protein (IsiA) accumulated to high levels within 48 h of the onset of iron deprivation, reaching a molar ratio of ~42 IsiA : Photosystem I in Synechococcus PCC 7942 and ~12 IsiA : Photosystem I in Synechocystis PCC 6803. Concomitantly the iron-rich complexes Cytochrome b6f and Photosystem I declined in abundance, leading to a decrease in the Photosystem I : Photosystem II ratio. Chlorophyll fluorescence analyses showed a drop in electron transport per Photosystem II in Synechococcus, but not in Synechocystis after iron depletion. We found no evidence that the accumulated IsiA contributes to light capture by Photosystem II complexes.

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Published date: 19 March 2013
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Organisations: Ocean and Earth Science

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Local EPrints ID: 350192
URI: http://eprints.soton.ac.uk/id/eprint/350192
ISSN: 1932-6203
PURE UUID: 962ed35d-7a20-4035-a5ab-7776e8428187

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Date deposited: 19 Mar 2013 12:51
Last modified: 08 Jan 2022 15:05

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Contributors

Author: J.M. Fraser
Author: S.E. Tulk
Author: D.A. Campbell
Author: T.S. Bibby
Author: A.M. Cockshutt

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