BCG vaccination induces different cytokine profiles following infant BCG vaccination in the UK and Malawi
BCG vaccination induces different cytokine profiles following infant BCG vaccination in the UK and Malawi
BACKGROUND: BCG vaccination of infants is thought to provide good protection in all settings. This study investigated whether Malawian infants made weaker responses across a cytokine panel after BCG vaccination, compared with UK infants.
METHODS: Diluted whole-blood samples were cultured with Mycobacterium tuberculosis purified protein derivative for 6 days from BCG-vaccinated infants 3 months (n = 40 Malawi, 28 UK) and 12 months (n = 34 Malawi, 26 UK) after vaccination, and also from UK unvaccinated infants (n = 9 at 3 months, n = 10 at 12 months). Forty-two cytokines were measured in supernatants using a multiplex bead array assay. Principal component analysis was used to summarize the overall patterns in cytokine responses.
RESULTS: We found differences in median responses in 27 of the 42 cytokines: 7 higher in the UK and 20 higher in Malawi. The cytokines with higher responses in the UK were all T helper 1 related. The cytokines with higher responses in Malawi included innate proinflammatory cytokines, regulatory cytokines, interleukin 17, T helper 2 cytokines, chemokines, and growth factors. Principal component analysis separated the BCG-vaccinated infants from Malawi from the UK vaccinated infants and from the unvaccinated infants.
CONCLUSIONS: Malawian infants make cytokine responses following BCG vaccination, but the cytokine profile is different from that in the UK. The different biosignatures following BCG vaccination in the 2 settings may indicate variability in the protective efficacy of infant BCG vaccination.
1075-1085
Lalor, M.K.
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Floyd, S.
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Gorak-Stolinska, P.
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Ben-Smith, A.
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Weir, R.E.
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Smith, S.G.
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Newport, M.J.
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Blitz, R.
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Mvula, H.
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Branson, K.
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McGrath, N.
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Crampin, A.C.
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Fine, P.E.
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Dockrell, H.M.
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Lalor, M.K.
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Floyd, S.
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Gorak-Stolinska, P.
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Ben-Smith, A.
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Weir, R.E.
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Smith, S.G.
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Newport, M.J.
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Blitz, R.
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Mvula, H.
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Branson, K.
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McGrath, N.
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Crampin, A.C.
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Fine, P.E.
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Dockrell, H.M.
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Lalor, M.K., Floyd, S., Gorak-Stolinska, P., Ben-Smith, A., Weir, R.E., Smith, S.G., Newport, M.J., Blitz, R., Mvula, H., Branson, K., McGrath, N., Crampin, A.C., Fine, P.E. and Dockrell, H.M.
(2011)
BCG vaccination induces different cytokine profiles following infant BCG vaccination in the UK and Malawi.
The Journal of Infectious Diseases, 204 (7), .
(doi:10.1093/infdis/jir515).
(PMID:21881123)
Abstract
BACKGROUND: BCG vaccination of infants is thought to provide good protection in all settings. This study investigated whether Malawian infants made weaker responses across a cytokine panel after BCG vaccination, compared with UK infants.
METHODS: Diluted whole-blood samples were cultured with Mycobacterium tuberculosis purified protein derivative for 6 days from BCG-vaccinated infants 3 months (n = 40 Malawi, 28 UK) and 12 months (n = 34 Malawi, 26 UK) after vaccination, and also from UK unvaccinated infants (n = 9 at 3 months, n = 10 at 12 months). Forty-two cytokines were measured in supernatants using a multiplex bead array assay. Principal component analysis was used to summarize the overall patterns in cytokine responses.
RESULTS: We found differences in median responses in 27 of the 42 cytokines: 7 higher in the UK and 20 higher in Malawi. The cytokines with higher responses in the UK were all T helper 1 related. The cytokines with higher responses in Malawi included innate proinflammatory cytokines, regulatory cytokines, interleukin 17, T helper 2 cytokines, chemokines, and growth factors. Principal component analysis separated the BCG-vaccinated infants from Malawi from the UK vaccinated infants and from the unvaccinated infants.
CONCLUSIONS: Malawian infants make cytokine responses following BCG vaccination, but the cytokine profile is different from that in the UK. The different biosignatures following BCG vaccination in the 2 settings may indicate variability in the protective efficacy of infant BCG vaccination.
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e-pub ahead of print date: 1 October 2011
Organisations:
Primary Care & Population Sciences, Faculty of Social, Human and Mathematical Sciences
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Local EPrints ID: 350259
URI: http://eprints.soton.ac.uk/id/eprint/350259
ISSN: 0022-1899
PURE UUID: 9d044cc6-ddcc-4663-a5eb-d3a64956dda5
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Date deposited: 20 Mar 2013 14:20
Last modified: 15 Mar 2024 03:46
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Contributors
Author:
M.K. Lalor
Author:
S. Floyd
Author:
P. Gorak-Stolinska
Author:
A. Ben-Smith
Author:
R.E. Weir
Author:
S.G. Smith
Author:
M.J. Newport
Author:
R. Blitz
Author:
H. Mvula
Author:
K. Branson
Author:
A.C. Crampin
Author:
P.E. Fine
Author:
H.M. Dockrell
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