Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique
Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique
We discuss a hyperspectral darkfield microscopy technique capable of imaging single nanoparticles at biologically compatible (<0.5 W/cm2) illumination conditions. The microscope was tested on an array of lithographically produced gold nanorod antennas and on colloidal gold nanorods deposited on a glass substrate. As a test for in-vitro imaging capabilities, we studied the uptake of gold nanorods by DC2.4 dendritic cells.
nanoparticles, darkfield microscopy, hyperspectral, live-cell imaging
859501-859501
Fairbairn, Natasha
0dda470b-3803-48be-a254-08b8aee1be38
Fernandes, Rute
848317d8-1eb0-48ad-aefc-012ab19d9f52
Carter, Rachel
840a775a-867e-42ba-a7cb-d131c9fdb9c6
Elliot, Timothy J.
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
22 February 2013
Fairbairn, Natasha
0dda470b-3803-48be-a254-08b8aee1be38
Fernandes, Rute
848317d8-1eb0-48ad-aefc-012ab19d9f52
Carter, Rachel
840a775a-867e-42ba-a7cb-d131c9fdb9c6
Elliot, Timothy J.
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Fairbairn, Natasha, Fernandes, Rute, Carter, Rachel, Elliot, Timothy J., Kanaras, Antonios G. and Muskens, Otto L.
(2013)
Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique.
In Proceedings SPIE 8595: Colloidal Nanocrystals for Biomedical Applications VIII.
SPIE.
.
(doi:10.1117/12.981941).
Record type:
Conference or Workshop Item
(Paper)
Abstract
We discuss a hyperspectral darkfield microscopy technique capable of imaging single nanoparticles at biologically compatible (<0.5 W/cm2) illumination conditions. The microscope was tested on an array of lithographically produced gold nanorod antennas and on colloidal gold nanorods deposited on a glass substrate. As a test for in-vitro imaging capabilities, we studied the uptake of gold nanorods by DC2.4 dendritic cells.
Text
FairbairnSPIE2013.pdf
- Accepted Manuscript
More information
e-pub ahead of print date: 2 February 2013
Published date: 22 February 2013
Additional Information:
Copyright 2013 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Venue - Dates:
SPIE Photonics West 2013, , San Francisco, United States, 2013-02-02 - 2013-02-07
Keywords:
nanoparticles, darkfield microscopy, hyperspectral, live-cell imaging
Organisations:
Quantum, Light & Matter Group
Identifiers
Local EPrints ID: 350417
URI: http://eprints.soton.ac.uk/id/eprint/350417
PURE UUID: af5dc9a1-8bcd-4a5c-b5eb-dc1130945ba9
Catalogue record
Date deposited: 26 Mar 2013 15:17
Last modified: 15 Mar 2024 03:34
Export record
Altmetrics
Contributors
Author:
Natasha Fairbairn
Author:
Rute Fernandes
Author:
Rachel Carter
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
