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Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique

Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique
Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique
We discuss a hyperspectral darkfield microscopy technique capable of imaging single nanoparticles at biologically compatible (<0.5 W/cm2) illumination conditions. The microscope was tested on an array of lithographically produced gold nanorod antennas and on colloidal gold nanorods deposited on a glass substrate. As a test for in-vitro imaging capabilities, we studied the uptake of gold nanorods by DC2.4 dendritic cells.
nanoparticles, darkfield microscopy, hyperspectral, live-cell imaging
859501-859501
SPIE: the International Society for Optics and Photonics
Fairbairn, Natasha
0dda470b-3803-48be-a254-08b8aee1be38
Fernandes, Rute
848317d8-1eb0-48ad-aefc-012ab19d9f52
Carter, Rachel
840a775a-867e-42ba-a7cb-d131c9fdb9c6
Elliot, Timothy J.
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9
Fairbairn, Natasha
0dda470b-3803-48be-a254-08b8aee1be38
Fernandes, Rute
848317d8-1eb0-48ad-aefc-012ab19d9f52
Carter, Rachel
840a775a-867e-42ba-a7cb-d131c9fdb9c6
Elliot, Timothy J.
16670fa8-c2f9-477a-91df-7c9e5b453e0e
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Muskens, Otto L.
2284101a-f9ef-4d79-8951-a6cda5bfc7f9

Fairbairn, Natasha, Fernandes, Rute, Carter, Rachel, Elliot, Timothy J., Kanaras, Antonios G. and Muskens, Otto L. (2013) Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique In Proceedings SPIE 8595: Colloidal Nanocrystals for Biomedical Applications VIII. SPIE: the International Society for Optics and Photonics., p. 859501. (doi:10.1117/12.981941).

Record type: Conference or Workshop Item (Paper)

Abstract

We discuss a hyperspectral darkfield microscopy technique capable of imaging single nanoparticles at biologically compatible (<0.5 W/cm2) illumination conditions. The microscope was tested on an array of lithographically produced gold nanorod antennas and on colloidal gold nanorods deposited on a glass substrate. As a test for in-vitro imaging capabilities, we studied the uptake of gold nanorods by DC2.4 dendritic cells.

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Published date: 22 February 2013
Additional Information: Copyright 2013 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Venue - Dates: SPIE Photonics West, 2013-02-22
Keywords: nanoparticles, darkfield microscopy, hyperspectral, live-cell imaging
Organisations: Quantum, Light & Matter Group

Identifiers

Local EPrints ID: 350417
URI: http://eprints.soton.ac.uk/id/eprint/350417
PURE UUID: af5dc9a1-8bcd-4a5c-b5eb-dc1130945ba9
ORCID for Timothy J. Elliot: ORCID iD orcid.org/0000-0003-1097-0222

Catalogue record

Date deposited: 26 Mar 2013 15:17
Last modified: 18 Jul 2017 04:34

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Contributors

Author: Natasha Fairbairn
Author: Rute Fernandes
Author: Rachel Carter
Author: Otto L. Muskens

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