Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique


Fairbairn, Natasha, Fernandes, Rute, Carter, Rachel, Elliot, Timothy J., Kanaras, Antonios G. and Muskens, Otto L. (2013) Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique In Proceedings SPIE 8595: Colloidal Nanocrystals for Biomedical Applications VIII. SPIE: the International Society for Optics and Photonics., p. 859501. (doi:10.1117/12.981941).

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Description/Abstract

We discuss a hyperspectral darkfield microscopy technique capable of imaging single nanoparticles at biologically compatible (<0.5 W/cm2) illumination conditions. The microscope was tested on an array of lithographically produced gold nanorod antennas and on colloidal gold nanorods deposited on a glass substrate. As a test for in-vitro imaging capabilities, we studied the uptake of gold nanorods by DC2.4 dendritic cells.

Item Type: Conference or Workshop Item (Paper)
Digital Object Identifier (DOI): doi:10.1117/12.981941
Additional Information: Copyright 2013 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Venue - Dates: SPIE Photonics West, 2013-02-22
Keywords: nanoparticles, darkfield microscopy, hyperspectral, live-cell imaging
Subjects: Q Science > QC Physics
Organisations: Quantum, Light & Matter Group
ePrint ID: 350417
Date :
Date Event
22 February 2013Published
Date Deposited: 26 Mar 2013 15:17
Last Modified: 17 Apr 2017 15:48
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/350417

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