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High resolution synchrotron imaging of wheat root hairs growing in soil and image based modelling of phosphate uptake

High resolution synchrotron imaging of wheat root hairs growing in soil and image based modelling of phosphate uptake
High resolution synchrotron imaging of wheat root hairs growing in soil and image based modelling of phosphate uptake
Root hairs are known to be highly important for uptake of sparingly soluble nutrients, particularly in nutrient deficient soils. Development of increasingly sophisticated mathematical models has allowed uptake characteristics to be quantified. However, modelling has been constrained by a lack of methods for imaging live root hairs growing in real soils.

We developed a plant growth protocol and used Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM) to uncover the 3D interactions of root hairs in real soil. We developed a model of phosphate uptake by root hairs based directly on the geometry of hairs and associated soil pores as revealed by imaging.

Previous modelling studies found that root hairs dominate phosphate uptake. By contrast, our study suggests that hairs and roots contribute equally. We show that uptake by hairs is more localised than by roots and strongly dependent on root hair and aggregate orientation.

The ability to image hair-soil interactions enables a step change in modelling approaches, allowing a more realistic treatment of processes at the scale of individual root hairs in soil pores.
3D reconstruction, imaging, root hair, x-ray CT, phosphate, modelling
0028-646X
1023-1029
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Daly, Keith R.
64f85c2e-2562-44df-9cb8-1be7fbc7e74c
Gostling, Neil J.
4840aa40-cb6c-4112-a0b9-694a869523fc
Jones, Davey L.
c8e739a8-07d9-4c32-a0d2-e95c7754d168
Talboys, Peter
605a39b8-92b2-475d-8071-2dd136b81615
Pinzer, Bernd R.
748f19a2-402d-4c45-9d08-323c06ce6e54
Boardman, Richard
5818d677-5732-4e8a-a342-7164dbb10df1
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe
Keyes, Samuel
ed3ee62b-e257-4b92-922c-023b232e8145
Daly, Keith R.
64f85c2e-2562-44df-9cb8-1be7fbc7e74c
Gostling, Neil J.
4840aa40-cb6c-4112-a0b9-694a869523fc
Jones, Davey L.
c8e739a8-07d9-4c32-a0d2-e95c7754d168
Talboys, Peter
605a39b8-92b2-475d-8071-2dd136b81615
Pinzer, Bernd R.
748f19a2-402d-4c45-9d08-323c06ce6e54
Boardman, Richard
5818d677-5732-4e8a-a342-7164dbb10df1
Sinclair, Ian
6005f6c1-f478-434e-a52d-d310c18ade0d
Marchant, Alan
3e54d51c-53b0-4df0-b428-2e73b071ee8e
Roose, Tiina
3581ab5b-71e1-4897-8d88-59f13f3bccfe

Keyes, Samuel, Daly, Keith R., Gostling, Neil J., Jones, Davey L., Talboys, Peter, Pinzer, Bernd R., Boardman, Richard, Sinclair, Ian, Marchant, Alan and Roose, Tiina (2013) High resolution synchrotron imaging of wheat root hairs growing in soil and image based modelling of phosphate uptake. New Phytologist, 198 (4), 1023-1029. (doi:10.1111/nph.12294). (PMID:23600607)

Record type: Article

Abstract

Root hairs are known to be highly important for uptake of sparingly soluble nutrients, particularly in nutrient deficient soils. Development of increasingly sophisticated mathematical models has allowed uptake characteristics to be quantified. However, modelling has been constrained by a lack of methods for imaging live root hairs growing in real soils.

We developed a plant growth protocol and used Synchrotron Radiation X-ray Tomographic Microscopy (SRXTM) to uncover the 3D interactions of root hairs in real soil. We developed a model of phosphate uptake by root hairs based directly on the geometry of hairs and associated soil pores as revealed by imaging.

Previous modelling studies found that root hairs dominate phosphate uptake. By contrast, our study suggests that hairs and roots contribute equally. We show that uptake by hairs is more localised than by roots and strongly dependent on root hair and aggregate orientation.

The ability to image hair-soil interactions enables a step change in modelling approaches, allowing a more realistic treatment of processes at the scale of individual root hairs in soil pores.

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More information

e-pub ahead of print date: 22 April 2013
Published date: June 2013
Keywords: 3D reconstruction, imaging, root hair, x-ray CT, phosphate, modelling
Organisations: Mathematical Sciences, Bioengineering Group, Centre for Biological Sciences

Identifiers

Local EPrints ID: 350444
URI: https://eprints.soton.ac.uk/id/eprint/350444
ISSN: 0028-646X
PURE UUID: 9f508757-367c-456c-ae7b-6fe5897a2408
ORCID for Samuel Keyes: ORCID iD orcid.org/0000-0002-4129-2228
ORCID for Tiina Roose: ORCID iD orcid.org/0000-0001-8710-1063

Catalogue record

Date deposited: 02 Oct 2014 15:57
Last modified: 06 Jun 2018 12:36

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