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A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guérin immunisation in children and adults

A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guérin immunisation in children and adults
A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guérin immunisation in children and adults
BCG vaccine is one of the most commonly-administered vaccines worldwide. Studies suggest the protective efficacy of BCG against TB is better for children than for adults. One potential explanation is that BCG induces a better protective immune response in children. Twenty six children and adults were immunised with BCG. The proportion of Th1-cytokine-producing mycobacterial-specific T cells, and the concentrations of secreted cytokines, were measured before and 10 weeks after BCG immunisation. A significant increase in the proportion of mycobacterial-specific cytokine-producing T cells was observed in both age groups. After BCG immunisation, children and adults had comparable proportions of mycobacterial-specific polyfunctional CD4 T cells when measured relative to the total number of CD4 T cells. However, relative to the subset of Th-1-cytokine-producing CD4 T cells, the proportion of polyfunctional cells was greater in children. Concentrations of secreted cytokines were comparable in children and adults. These findings suggest that the mycobacterial-specific cell-mediated immune response induced by BCG immunisation in children and adults is similar. The implication of a shift to a more polyfunctional immune response within the Th1-cytokine-producing CD4 T cells in children is uncertain as this aspect of the immune response has not been assessed as a potential correlate of protection against TB.

1932-6203
e37535
Ritz, N
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Strach, M
94c852ed-55d1-48cc-b5ce-8db56337a14e
Yau, C
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Dutta, B
c3cf0897-8c3a-4aa9-a710-e5969083fba2
Tebruegge, Marc
2c3dff22-0b5f-48a7-bb36-ce323705f74a
Connell, T
5737997c-b776-4fa0-8213-fc2ac51cb683
Hanekom, WA
4532ffa4-912f-499a-99ac-feffb2d4213b
Britton, WJ
dc731051-6b20-4c02-bda1-003c603833cf
Robins-Browne, R
754c62a4-385d-4b6d-a53d-4e83d0a51383
Curtis, N
1b87d286-995f-4fea-a008-aa5dd7f634b7
Ritz, N
01e4551a-bf95-4ff0-a137-09997a70d503
Strach, M
94c852ed-55d1-48cc-b5ce-8db56337a14e
Yau, C
0c1d2514-4815-435d-b02a-98731d0d6d21
Dutta, B
c3cf0897-8c3a-4aa9-a710-e5969083fba2
Tebruegge, Marc
2c3dff22-0b5f-48a7-bb36-ce323705f74a
Connell, T
5737997c-b776-4fa0-8213-fc2ac51cb683
Hanekom, WA
4532ffa4-912f-499a-99ac-feffb2d4213b
Britton, WJ
dc731051-6b20-4c02-bda1-003c603833cf
Robins-Browne, R
754c62a4-385d-4b6d-a53d-4e83d0a51383
Curtis, N
1b87d286-995f-4fea-a008-aa5dd7f634b7

Ritz, N, Strach, M, Yau, C, Dutta, B, Tebruegge, Marc, Connell, T, Hanekom, WA, Britton, WJ, Robins-Browne, R and Curtis, N (2012) A comparative analysis of polyfunctional T cells and secreted cytokines induced by Bacille Calmette-Guérin immunisation in children and adults. PLoS ONE, 7 (7), e37535. (doi:10.1371/journal.pone.0037535). (PMID:22829867)

Record type: Article

Abstract

BCG vaccine is one of the most commonly-administered vaccines worldwide. Studies suggest the protective efficacy of BCG against TB is better for children than for adults. One potential explanation is that BCG induces a better protective immune response in children. Twenty six children and adults were immunised with BCG. The proportion of Th1-cytokine-producing mycobacterial-specific T cells, and the concentrations of secreted cytokines, were measured before and 10 weeks after BCG immunisation. A significant increase in the proportion of mycobacterial-specific cytokine-producing T cells was observed in both age groups. After BCG immunisation, children and adults had comparable proportions of mycobacterial-specific polyfunctional CD4 T cells when measured relative to the total number of CD4 T cells. However, relative to the subset of Th-1-cytokine-producing CD4 T cells, the proportion of polyfunctional cells was greater in children. Concentrations of secreted cytokines were comparable in children and adults. These findings suggest that the mycobacterial-specific cell-mediated immune response induced by BCG immunisation in children and adults is similar. The implication of a shift to a more polyfunctional immune response within the Th1-cytokine-producing CD4 T cells in children is uncertain as this aspect of the immune response has not been assessed as a potential correlate of protection against TB.

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Published date: July 2012
Organisations: Faculty of Medicine

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Local EPrints ID: 350866
URI: http://eprints.soton.ac.uk/id/eprint/350866
ISSN: 1932-6203
PURE UUID: 4ec2140c-1f9c-4a5f-9c69-97caf5425dcf

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Date deposited: 09 Apr 2013 13:01
Last modified: 02 Dec 2019 20:52

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Contributors

Author: N Ritz
Author: M Strach
Author: C Yau
Author: B Dutta
Author: Marc Tebruegge
Author: T Connell
Author: WA Hanekom
Author: WJ Britton
Author: R Robins-Browne
Author: N Curtis

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