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CD36 and SR-BI are involved in cellular uptake of provitamin A carotenoids by Caco-2 and HEK Cells, and some of their genetic variants are associated with plasma concentrations of these micronutrients in humans.

CD36 and SR-BI are involved in cellular uptake of provitamin A carotenoids by Caco-2 and HEK Cells, and some of their genetic variants are associated with plasma concentrations of these micronutrients in humans.
CD36 and SR-BI are involved in cellular uptake of provitamin A carotenoids by Caco-2 and HEK Cells, and some of their genetic variants are associated with plasma concentrations of these micronutrients in humans.
Scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36) have been involved in cellular uptake of some provitamin A carotenoids. However, data are incomplete (e.g., there are no data on ?-carotene), and it is not known whether genetic variants in their encoding genes can affect provitamin A carotenoid status. The objectives were 1) to assess the involvement of these scavenger receptors in cellular uptake of the main provitamin A carotenoids (i.e., ?-carotene, ?-carotene, and ?-cryptoxanthin) as well as that of preformed vitamin A (i.e., retinol) and 2) to investigate the contribution of genetic variations in genes encoding these proteins to interindividual variations in plasma concentrations of provitamin A carotenoids. The involvement of SR-BI and CD36 in carotenoids and retinol cellular uptake was investigated in Caco-2 and human embryonic kidney (HEK) cell lines. The involvement of scavenger receptor class B type I (SCARB1) and CD36 genetic variants on plasma concentrations of provitamin A carotenoids was assessed by association studies in 3 independent populations. Cell experiments suggested the involvement of both proteins in cellular uptake of provitamin A carotenoids but not in that of retinol. Association studies showed that several plasma provitamin A carotenoid concentrations were significantly different (P < 0.0083) between participants who bore different genotypes at single nucleotide polymorphisms and haplotypes in CD36 and SCARB1. In conclusion, SR-BI and CD36 are involved in cellular uptake of provitamin A carotenoids, and genetic variations in their encoding genes may modulate plasma concentrations of provitamin A carotenoids at a population level.

0022-3166
448-456
Borel, P.
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Lietz, G.
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Goncalves, A.
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Szabo de Edelenyi, F.
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Lecompte, S.
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Curtis, P.
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Goumidi, L.
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Caslake, M.J.
2f6f6ea6-d99a-45d1-b543-367af7539054
Miles, Elizabeth A.
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Packard, C.
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Calder, P.C.
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Mathers, J.C .
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Minihane, A.M.
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Tourniaire, F.
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Kesse-Guyot, E.
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Galan, P.
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Hercberg, S.
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Breidenassel, C.
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González Gross, M.
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Moussa, M.
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Meirhaeghe, A.
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Reboul, E.
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Borel, P.
a1bb985c-c637-4e20-9387-8d05d1fc9a3f
Lietz, G.
858cba85-d01d-44ad-bea3-dbf84a572173
Goncalves, A.
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Szabo de Edelenyi, F.
cdd5f912-977b-4676-add5-8546a46df93a
Lecompte, S.
76a7843c-271c-430d-828b-5dfdd0901c20
Curtis, P.
b8027827-e054-47b9-a267-2240e2dde3fc
Goumidi, L.
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Caslake, M.J.
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Miles, Elizabeth A.
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Packard, C.
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Calder, P.C.
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Mathers, J.C .
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Minihane, A.M.
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Tourniaire, F.
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Kesse-Guyot, E.
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Galan, P.
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Hercberg, S.
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Breidenassel, C.
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González Gross, M.
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Moussa, M.
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Meirhaeghe, A.
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Reboul, E.
47799c96-4f27-450f-a3d8-4b24ea190f3a

Borel, P., Lietz, G., Goncalves, A., Szabo de Edelenyi, F., Lecompte, S., Curtis, P., Goumidi, L., Caslake, M.J., Miles, Elizabeth A., Packard, C., Calder, P.C., Mathers, J.C ., Minihane, A.M., Tourniaire, F., Kesse-Guyot, E., Galan, P., Hercberg, S., Breidenassel, C., González Gross, M., Moussa, M., Meirhaeghe, A. and Reboul, E. (2013) CD36 and SR-BI are involved in cellular uptake of provitamin A carotenoids by Caco-2 and HEK Cells, and some of their genetic variants are associated with plasma concentrations of these micronutrients in humans. Journal of Nutrition, 143 (4), 448-456. (doi:10.3945/?jn.112.172734). (PMID:23427331)

Record type: Article

Abstract

Scavenger receptor class B type I (SR-BI) and cluster determinant 36 (CD36) have been involved in cellular uptake of some provitamin A carotenoids. However, data are incomplete (e.g., there are no data on ?-carotene), and it is not known whether genetic variants in their encoding genes can affect provitamin A carotenoid status. The objectives were 1) to assess the involvement of these scavenger receptors in cellular uptake of the main provitamin A carotenoids (i.e., ?-carotene, ?-carotene, and ?-cryptoxanthin) as well as that of preformed vitamin A (i.e., retinol) and 2) to investigate the contribution of genetic variations in genes encoding these proteins to interindividual variations in plasma concentrations of provitamin A carotenoids. The involvement of SR-BI and CD36 in carotenoids and retinol cellular uptake was investigated in Caco-2 and human embryonic kidney (HEK) cell lines. The involvement of scavenger receptor class B type I (SCARB1) and CD36 genetic variants on plasma concentrations of provitamin A carotenoids was assessed by association studies in 3 independent populations. Cell experiments suggested the involvement of both proteins in cellular uptake of provitamin A carotenoids but not in that of retinol. Association studies showed that several plasma provitamin A carotenoid concentrations were significantly different (P < 0.0083) between participants who bore different genotypes at single nucleotide polymorphisms and haplotypes in CD36 and SCARB1. In conclusion, SR-BI and CD36 are involved in cellular uptake of provitamin A carotenoids, and genetic variations in their encoding genes may modulate plasma concentrations of provitamin A carotenoids at a population level.

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Published date: April 2013
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 350880
URI: http://eprints.soton.ac.uk/id/eprint/350880
ISSN: 0022-3166
PURE UUID: e8cb31e9-d62e-4a36-a1aa-17e0e03fb08e
ORCID for Elizabeth A. Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for P.C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 09 Apr 2013 14:05
Last modified: 28 Apr 2022 01:39

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Contributors

Author: P. Borel
Author: G. Lietz
Author: A. Goncalves
Author: F. Szabo de Edelenyi
Author: S. Lecompte
Author: P. Curtis
Author: L. Goumidi
Author: M.J. Caslake
Author: C. Packard
Author: P.C. Calder ORCID iD
Author: J.C . Mathers
Author: A.M. Minihane
Author: F. Tourniaire
Author: E. Kesse-Guyot
Author: P. Galan
Author: S. Hercberg
Author: C. Breidenassel
Author: M. González Gross
Author: M. Moussa
Author: A. Meirhaeghe
Author: E. Reboul

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