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Membrane protrusion coarsening and nanotubulation within giant unilamellar vesicles

Membrane protrusion coarsening and nanotubulation within giant unilamellar vesicles
Membrane protrusion coarsening and nanotubulation within giant unilamellar vesicles
Hydrophobic side groups on a stimuli-responsive polymer, encapsulated within a single giant unilamellar vesicle, enable membrane attachment during compartment formation at elevated temperatures. We thermally modulated the vesicle through implementation of an IR laser via an optical fiber, enabling localized directed heating. Polymer-membrane interactions were monitored using confocal imaging techniques as subsequent membrane protrusions occurred and lipid nanotubes formed in response to the polymer hydrogel contraction. These nanotubes, bridging the vesicle membrane to the contracting hydrogel, were retained on the surface of the polymer compartment, where they were transformed into smaller vesicles in a process reminiscent of cellular endocytosis. This development of a synthetic vesicle system containing a stimuli-responsive polymer could lead to a new platform for studying inter/intramembrane transport through lipid nanotubes.
0002-7863
18046-18049
Węgrzyn, Ilona
b05fda84-5ba5-43d1-abd2-d866f3a75f6e
Jeffries, Gavin D.M.
2c844689-b127-44c7-a597-9ca3d3b10b3b
Nagel, Birgit
6d9be1ae-a8ed-4c4f-b812-2b1eb09ebf6f
Katterle, Martin
3f608351-fbbe-4360-89ee-3c5f31a37130
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Orwar, Owe
507d5098-e27d-4d04-ba76-a8173bdbf7ea
Jesorka, Aldo
2c99fa24-a8da-42fa-8847-fc5a689818e3
Węgrzyn, Ilona
b05fda84-5ba5-43d1-abd2-d866f3a75f6e
Jeffries, Gavin D.M.
2c844689-b127-44c7-a597-9ca3d3b10b3b
Nagel, Birgit
6d9be1ae-a8ed-4c4f-b812-2b1eb09ebf6f
Katterle, Martin
3f608351-fbbe-4360-89ee-3c5f31a37130
Gerrard, Simon R.
4ed7b304-42c2-497a-b00d-5ac8a1bedd24
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Orwar, Owe
507d5098-e27d-4d04-ba76-a8173bdbf7ea
Jesorka, Aldo
2c99fa24-a8da-42fa-8847-fc5a689818e3

Węgrzyn, Ilona, Jeffries, Gavin D.M., Nagel, Birgit, Katterle, Martin, Gerrard, Simon R., Brown, Tom, Orwar, Owe and Jesorka, Aldo (2011) Membrane protrusion coarsening and nanotubulation within giant unilamellar vesicles. Journal of the American Chemical Society, 133 (45), 18046-18049. (doi:10.1021/ja207536a). (PMID:21978148)

Record type: Article

Abstract

Hydrophobic side groups on a stimuli-responsive polymer, encapsulated within a single giant unilamellar vesicle, enable membrane attachment during compartment formation at elevated temperatures. We thermally modulated the vesicle through implementation of an IR laser via an optical fiber, enabling localized directed heating. Polymer-membrane interactions were monitored using confocal imaging techniques as subsequent membrane protrusions occurred and lipid nanotubes formed in response to the polymer hydrogel contraction. These nanotubes, bridging the vesicle membrane to the contracting hydrogel, were retained on the surface of the polymer compartment, where they were transformed into smaller vesicles in a process reminiscent of cellular endocytosis. This development of a synthetic vesicle system containing a stimuli-responsive polymer could lead to a new platform for studying inter/intramembrane transport through lipid nanotubes.

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More information

e-pub ahead of print date: 6 October 2011
Published date: 2011
Organisations: Organic Chemistry: SCF

Identifiers

Local EPrints ID: 351698
URI: http://eprints.soton.ac.uk/id/eprint/351698
ISSN: 0002-7863
PURE UUID: 2e1c1046-747c-49b2-a4b7-002544ecc52a

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Date deposited: 30 Apr 2013 10:31
Last modified: 14 Mar 2024 13:41

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Contributors

Author: Ilona Węgrzyn
Author: Gavin D.M. Jeffries
Author: Birgit Nagel
Author: Martin Katterle
Author: Simon R. Gerrard
Author: Tom Brown
Author: Owe Orwar
Author: Aldo Jesorka

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