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Lower maternal body condition during pregnancy affects skeletal muscle structure and glut-4 protein levels but not glucose tolerance in mature adult sheep

Lower maternal body condition during pregnancy affects skeletal muscle structure and glut-4 protein levels but not glucose tolerance in mature adult sheep
Lower maternal body condition during pregnancy affects skeletal muscle structure and glut-4 protein levels but not glucose tolerance in mature adult sheep
Suboptimal maternal nutrition and body composition are implicated in metabolic disease risk in adult offspring. We hypothesized that modest disruption of glucose homeostasis previously observed in young adult sheep offspring from ewes of a lower body condition score (BCS) would deteriorate with age, due to changes in skeletal muscle structure and insulin signaling mechanisms. Ewes were fed to achieve a lower (LBCS, n = 10) or higher (HBCS, n = 14) BCS before and during pregnancy. Baseline plasma glucose, glucose tolerance and basal glucose uptake into isolated muscle strips were similar in male offspring at 210 ± 4 weeks. Vastus total myofiber density (HBCS, 343 ± 15; LBCS, 294 ± 14 fibers/mm(2), P < .05) and fast myofiber density (HBCS, 226 ± 10; LBCS 194 ± 10 fibers/mm(2), P < .05), capillary to myofiber ratio (HBCS, 1.5 ± 0.1; LBCS 1.2 ± 0.1 capillary:myofiber, P < .05) were lower in LBCS offspring. Vastus protein levels of Akt1 were lower (83% ± 7% of HBCS, P < .05), and total glucose transporter 4 was increased (157% ± 6% of HBCS, P < .001) in LBCS offspring, Despite the reduction in total myofiber density in LBCS offspring, glucose tolerance was normal in mature adult life. However, such adaptations may lead to complications in metabolic control in an overabundant postnatal nutrient environment.
1933-7191
1144-1155
Costello, Paula M.
376e2438-9b17-4f27-bacd-c94fd50511f6
Hollis, Lisa J.
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Cripps, Roselle L.
74a77335-3c95-4bfd-9e0a-3d989fcd4f6e
Bearpark, Natasha
388934ca-1edb-4ffe-a2fb-d5f78fd70ebe
Patel, Harnish P.
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Sayer, Avan Aihie
22ab6f4d-9d83-4272-9f56-1c2787598509
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Hanson, Mark A.
525a8bc1-9e29-46fe-aead-73a5a9f69f56
Ozanne, Susan E.
bba0ebbc-1d8d-497c-ad46-518ca6e52f3a
Green, Lucy R.
1c5b6705-4ea6-4d39-a859-4ac9c559db31
Costello, Paula M.
376e2438-9b17-4f27-bacd-c94fd50511f6
Hollis, Lisa J.
2d6c91b6-f83a-451e-9a8a-cf1cbec6337f
Cripps, Roselle L.
74a77335-3c95-4bfd-9e0a-3d989fcd4f6e
Bearpark, Natasha
388934ca-1edb-4ffe-a2fb-d5f78fd70ebe
Patel, Harnish P.
df99f0d2-02d4-4cfe-89bb-6c9443f4aa9b
Sayer, Avan Aihie
22ab6f4d-9d83-4272-9f56-1c2787598509
Cooper, Cyrus
e05f5612-b493-4273-9b71-9e0ce32bdad6
Hanson, Mark A.
525a8bc1-9e29-46fe-aead-73a5a9f69f56
Ozanne, Susan E.
bba0ebbc-1d8d-497c-ad46-518ca6e52f3a
Green, Lucy R.
1c5b6705-4ea6-4d39-a859-4ac9c559db31

Costello, Paula M., Hollis, Lisa J., Cripps, Roselle L., Bearpark, Natasha, Patel, Harnish P., Sayer, Avan Aihie, Cooper, Cyrus, Hanson, Mark A., Ozanne, Susan E. and Green, Lucy R. (2013) Lower maternal body condition during pregnancy affects skeletal muscle structure and glut-4 protein levels but not glucose tolerance in mature adult sheep. Reproductive Sciences, 20 (20), 1144-1155. (doi:10.1177/1933719113477494). (PMID:23420826)

Record type: Article

Abstract

Suboptimal maternal nutrition and body composition are implicated in metabolic disease risk in adult offspring. We hypothesized that modest disruption of glucose homeostasis previously observed in young adult sheep offspring from ewes of a lower body condition score (BCS) would deteriorate with age, due to changes in skeletal muscle structure and insulin signaling mechanisms. Ewes were fed to achieve a lower (LBCS, n = 10) or higher (HBCS, n = 14) BCS before and during pregnancy. Baseline plasma glucose, glucose tolerance and basal glucose uptake into isolated muscle strips were similar in male offspring at 210 ± 4 weeks. Vastus total myofiber density (HBCS, 343 ± 15; LBCS, 294 ± 14 fibers/mm(2), P < .05) and fast myofiber density (HBCS, 226 ± 10; LBCS 194 ± 10 fibers/mm(2), P < .05), capillary to myofiber ratio (HBCS, 1.5 ± 0.1; LBCS 1.2 ± 0.1 capillary:myofiber, P < .05) were lower in LBCS offspring. Vastus protein levels of Akt1 were lower (83% ± 7% of HBCS, P < .05), and total glucose transporter 4 was increased (157% ± 6% of HBCS, P < .001) in LBCS offspring, Despite the reduction in total myofiber density in LBCS offspring, glucose tolerance was normal in mature adult life. However, such adaptations may lead to complications in metabolic control in an overabundant postnatal nutrient environment.

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e-pub ahead of print date: 18 February 2013
Published date: October 2013
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 352064
URI: http://eprints.soton.ac.uk/id/eprint/352064
ISSN: 1933-7191
PURE UUID: 7457a429-29f4-4534-8b84-f62ab926fe6d
ORCID for Cyrus Cooper: ORCID iD orcid.org/0000-0003-3510-0709

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Date deposited: 01 May 2013 14:30
Last modified: 26 Jan 2022 02:34

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Contributors

Author: Paula M. Costello
Author: Lisa J. Hollis
Author: Roselle L. Cripps
Author: Natasha Bearpark
Author: Harnish P. Patel
Author: Avan Aihie Sayer
Author: Cyrus Cooper ORCID iD
Author: Mark A. Hanson
Author: Susan E. Ozanne
Author: Lucy R. Green

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