The use of culture-independent tools to characterize bacteria in endo-tracheal aspirates from pre-term infants at risk of bronchopulmonary dysplasia
The use of culture-independent tools to characterize bacteria in endo-tracheal aspirates from pre-term infants at risk of bronchopulmonary dysplasia
Although premature infants are increasingly surviving the neonatal period, up to one-third develop bronchopulmonary dysplasia (BPD). Despite evidence that bacterial colonization of the neonatal respiratory tract by certain bacteria may be a risk factor in BPD development, little is known about the role these bacteria play. The aim of this study was to investigate the use of culture-independent molecular profiling methodologies to identify potential etiological agents in neonatal airway secretions. This study used terminal restriction fragment length polymorphism (T-RFLP) and clone sequence analyses to characterize bacterial species in endo-tracheal (ET) aspirates from eight intubated pre-term infants. A wide range of different bacteria was identified in the samples. Forty-seven T-RF band lengths were resolved in the sample set, with a range of 0-15 separate species in each patient. Clone sequence analyses confirmed the identity of individual species detected by T-RFLP. We speculate that the identification of known opportunistic pathogens including S. aureus, Enterobacter sp., Moraxella catarrhalis, Pseudomonas aeruginosa and Streptococcus sp., within the airways of pre-term infants, might be causally related to the subsequent development of BPD. Further, we suggest that culture-independent techniques, such as T-RFLP, hold important potential for the characterization of neonatal conditions, such as BPD.
333-337
Stressmann, F.A.
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Connett, G.J.
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Goss, K.
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Kollamparambil, T.G.
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Patel, N.
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Payne, M.S.
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Puddy, V.
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Legg, J.
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Bruce, K.D.
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Rogers, G.B.
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2 February 2010
Stressmann, F.A.
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Connett, G.J.
55d5676c-90d8-46bf-a508-62eded276516
Goss, K.
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Kollamparambil, T.G.
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Patel, N.
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Payne, M.S.
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Puddy, V.
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Legg, J.
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Bruce, K.D.
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Rogers, G.B.
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Stressmann, F.A., Connett, G.J., Goss, K., Kollamparambil, T.G., Patel, N., Payne, M.S., Puddy, V., Legg, J., Bruce, K.D. and Rogers, G.B.
(2010)
The use of culture-independent tools to characterize bacteria in endo-tracheal aspirates from pre-term infants at risk of bronchopulmonary dysplasia.
Journal of Perinatal Medicine, 38 (3), .
(doi:10.1515/JPM.2010.026).
(PMID:20121490)
Abstract
Although premature infants are increasingly surviving the neonatal period, up to one-third develop bronchopulmonary dysplasia (BPD). Despite evidence that bacterial colonization of the neonatal respiratory tract by certain bacteria may be a risk factor in BPD development, little is known about the role these bacteria play. The aim of this study was to investigate the use of culture-independent molecular profiling methodologies to identify potential etiological agents in neonatal airway secretions. This study used terminal restriction fragment length polymorphism (T-RFLP) and clone sequence analyses to characterize bacterial species in endo-tracheal (ET) aspirates from eight intubated pre-term infants. A wide range of different bacteria was identified in the samples. Forty-seven T-RF band lengths were resolved in the sample set, with a range of 0-15 separate species in each patient. Clone sequence analyses confirmed the identity of individual species detected by T-RFLP. We speculate that the identification of known opportunistic pathogens including S. aureus, Enterobacter sp., Moraxella catarrhalis, Pseudomonas aeruginosa and Streptococcus sp., within the airways of pre-term infants, might be causally related to the subsequent development of BPD. Further, we suggest that culture-independent techniques, such as T-RFLP, hold important potential for the characterization of neonatal conditions, such as BPD.
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Published date: 2 February 2010
Organisations:
Faculty of Medicine
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Local EPrints ID: 352166
URI: http://eprints.soton.ac.uk/id/eprint/352166
ISSN: 0300-5577
PURE UUID: ee296968-44cf-412f-8e1b-3bbcbb3197eb
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Date deposited: 07 May 2013 12:56
Last modified: 15 Mar 2024 04:02
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Author:
F.A. Stressmann
Author:
G.J. Connett
Author:
K. Goss
Author:
T.G. Kollamparambil
Author:
N. Patel
Author:
M.S. Payne
Author:
V. Puddy
Author:
J. Legg
Author:
K.D. Bruce
Author:
G.B. Rogers
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