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A quantitative analysis of ureaplasma urealyticum and Ureaplasma parvum compared with host immune response in preterm neonates at risk of developing bronchopulmonary dysplasia

A quantitative analysis of ureaplasma urealyticum and Ureaplasma parvum compared with host immune response in preterm neonates at risk of developing bronchopulmonary dysplasia
A quantitative analysis of ureaplasma urealyticum and Ureaplasma parvum compared with host immune response in preterm neonates at risk of developing bronchopulmonary dysplasia
Multiplex, real-time PCR for the identification of Ureaplasma urealyticum and Ureaplasma parvum was performed on nucleic acids extracted from sequential endotracheal aspirates obtained from preterm neonates born at <29 weeks of gestation and ventilated for more than 48 h admitted to two level 3 neonatal intensive care units. Specimens were obtained shortly after birth and sequentially up until extubation. One hundred fifty-two specimens (93.8%) contained material suitable for analysis. Ureaplasma spp. were identified in 5 of 13 neonates studied. In most cases, the DNA load of the detected Ureaplasma species was low and decreased over time. In addition, changes in detectable Ureaplasma species DNA did not relate to changes in the inflammatory marker C-reactive protein (CRP) or respiratory status. All but two blood samples obtained at times of suspected sepsis were culture positive for other microorganisms; the species cultured were typically coagulase-negative staphylococci and were associated with increased levels of CRP (>10 mg/liter). This study was limited by the small number of patients examined and does not have the power to support or contradict the hypothesis that postnatal lung infection with Ureaplasma parvum is causally related to bronchopulmonary dysplasia (BPD) or adverse respiratory outcomes after preterm birth. However, in this study, increases in CRP levels were not associated with patients in whom Ureaplasma parvum was detected, in contrast to the detection of other bacterial species.
0095-1137
909-914
Payne, M.S.
8685e989-4f66-4e06-9c73-69a42fbd89c4
Goss, Kevin C.W.
d9ab6445-af10-40da-8b27-2f8ecf03c3d8
Connett, G.J.
55d5676c-90d8-46bf-a508-62eded276516
Legg, Julian P.
d794b6a3-768c-4986-b67f-bf9a30fe228e
Bruce, K.D.
1ded890b-addf-45bd-ba59-dbaedaeee931
Chalker, V.
270df151-8de1-4457-b59d-1146c17fbc5e
Payne, M.S.
8685e989-4f66-4e06-9c73-69a42fbd89c4
Goss, Kevin C.W.
d9ab6445-af10-40da-8b27-2f8ecf03c3d8
Connett, G.J.
55d5676c-90d8-46bf-a508-62eded276516
Legg, Julian P.
d794b6a3-768c-4986-b67f-bf9a30fe228e
Bruce, K.D.
1ded890b-addf-45bd-ba59-dbaedaeee931
Chalker, V.
270df151-8de1-4457-b59d-1146c17fbc5e

Payne, M.S., Goss, Kevin C.W., Connett, G.J., Legg, Julian P., Bruce, K.D. and Chalker, V. (2012) A quantitative analysis of ureaplasma urealyticum and Ureaplasma parvum compared with host immune response in preterm neonates at risk of developing bronchopulmonary dysplasia. Journal of Clinical Microbiology, 50 (3), 909-914. (doi:10.1128/JCM.06625-11). (PMID:22189123)

Record type: Article

Abstract

Multiplex, real-time PCR for the identification of Ureaplasma urealyticum and Ureaplasma parvum was performed on nucleic acids extracted from sequential endotracheal aspirates obtained from preterm neonates born at <29 weeks of gestation and ventilated for more than 48 h admitted to two level 3 neonatal intensive care units. Specimens were obtained shortly after birth and sequentially up until extubation. One hundred fifty-two specimens (93.8%) contained material suitable for analysis. Ureaplasma spp. were identified in 5 of 13 neonates studied. In most cases, the DNA load of the detected Ureaplasma species was low and decreased over time. In addition, changes in detectable Ureaplasma species DNA did not relate to changes in the inflammatory marker C-reactive protein (CRP) or respiratory status. All but two blood samples obtained at times of suspected sepsis were culture positive for other microorganisms; the species cultured were typically coagulase-negative staphylococci and were associated with increased levels of CRP (>10 mg/liter). This study was limited by the small number of patients examined and does not have the power to support or contradict the hypothesis that postnatal lung infection with Ureaplasma parvum is causally related to bronchopulmonary dysplasia (BPD) or adverse respiratory outcomes after preterm birth. However, in this study, increases in CRP levels were not associated with patients in whom Ureaplasma parvum was detected, in contrast to the detection of other bacterial species.

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e-pub ahead of print date: 21 December 2011
Published date: March 2012
Organisations: Faculty of Medicine

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Local EPrints ID: 352167
URI: http://eprints.soton.ac.uk/id/eprint/352167
ISSN: 0095-1137
PURE UUID: ce8b633d-fe0d-452b-86d3-fdf7124a901e
ORCID for G.J. Connett: ORCID iD orcid.org/0000-0003-1310-3239

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Date deposited: 07 May 2013 12:56
Last modified: 15 Oct 2019 00:23

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Contributors

Author: M.S. Payne
Author: Kevin C.W. Goss
Author: G.J. Connett ORCID iD
Author: Julian P. Legg
Author: K.D. Bruce
Author: V. Chalker

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