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Intracellular SERS nanoprobes for distinction of different neuronal cell types

Intracellular SERS nanoprobes for distinction of different neuronal cell types
Intracellular SERS nanoprobes for distinction of different neuronal cell types
Distinction between closely related and morphologically similar cells is difficult by conventional methods especially without labelling. Using nuclear targeted gold nanoparticles as intracellular probes we demonstrate the ability to distinguish between progenitor and differentiated cell types in a human neuroblastoma cell line using surface-enhanced Raman spectroscopy (SERS). SERS spectra from the whole cell area as well as only the nucleus were analysed using principal component analysis which allowed unambiguous distinction of the different cell types. SERS spectra from the nuclear region showed the developments during cellular differentiation by identifying an increase in DNA/RNA ratio and proteins transcribed. Our approach using nuclear targeted AuNPs and SERS imaging provides label-free and non-invasive characterization which can play a vital role in identifying cells types in bio-medical stem cell research.
gold nanoparticles, surface-enhanced raman scattering, cellular imaging, nuclear localization signal, cellular distinction, surface-enhanced raman, sers, biomedical sers
1530-6984
2463-2470
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Kuan, Wei-Li
f32d8025-2376-4408-a285-3f3d9fc11bdc
Barker, Roger
46d636f3-43ce-4591-970f-b897422965d9
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Kuan, Wei-Li
f32d8025-2376-4408-a285-3f3d9fc11bdc
Barker, Roger
46d636f3-43ce-4591-970f-b897422965d9
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9

Huefner, Anna, Kuan, Wei-Li, Barker, Roger and Mahajan, Sumeet (2013) Intracellular SERS nanoprobes for distinction of different neuronal cell types. Nano Letters, 13 (6), 2463-2470. (doi:10.1021/nl400448n).

Record type: Article

Abstract

Distinction between closely related and morphologically similar cells is difficult by conventional methods especially without labelling. Using nuclear targeted gold nanoparticles as intracellular probes we demonstrate the ability to distinguish between progenitor and differentiated cell types in a human neuroblastoma cell line using surface-enhanced Raman spectroscopy (SERS). SERS spectra from the whole cell area as well as only the nucleus were analysed using principal component analysis which allowed unambiguous distinction of the different cell types. SERS spectra from the nuclear region showed the developments during cellular differentiation by identifying an increase in DNA/RNA ratio and proteins transcribed. Our approach using nuclear targeted AuNPs and SERS imaging provides label-free and non-invasive characterization which can play a vital role in identifying cells types in bio-medical stem cell research.

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e-pub ahead of print date: 2 May 2013
Published date: 2013
Additional Information: Funded by EPSRC: Plasmon-enhanced spectroscopy and imaging inside cells (EP/H028757/1)
Keywords: gold nanoparticles, surface-enhanced raman scattering, cellular imaging, nuclear localization signal, cellular distinction, surface-enhanced raman, sers, biomedical sers
Organisations: Institute for Life Sciences, Organic Chemistry: SCF

Identifiers

Local EPrints ID: 352168
URI: http://eprints.soton.ac.uk/id/eprint/352168
ISSN: 1530-6984
PURE UUID: e5b71d3e-ba38-47e6-889c-fdf3d5e0aa0b
ORCID for Sumeet Mahajan: ORCID iD orcid.org/0000-0001-8923-6666

Catalogue record

Date deposited: 07 May 2013 13:06
Last modified: 21 Nov 2021 02:59

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Contributors

Author: Anna Huefner
Author: Wei-Li Kuan
Author: Roger Barker
Author: Sumeet Mahajan ORCID iD

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