Intracellular SERS nanoprobes for distinction of different neuronal cell types
Intracellular SERS nanoprobes for distinction of different neuronal cell types
Distinction between closely related and morphologically similar cells is difficult by conventional methods especially without labelling. Using nuclear targeted gold nanoparticles as intracellular probes we demonstrate the ability to distinguish between progenitor and differentiated cell types in a human neuroblastoma cell line using surface-enhanced Raman spectroscopy (SERS). SERS spectra from the whole cell area as well as only the nucleus were analysed using principal component analysis which allowed unambiguous distinction of the different cell types. SERS spectra from the nuclear region showed the developments during cellular differentiation by identifying an increase in DNA/RNA ratio and proteins transcribed. Our approach using nuclear targeted AuNPs and SERS imaging provides label-free and non-invasive characterization which can play a vital role in identifying cells types in bio-medical stem cell research.
gold nanoparticles, surface-enhanced raman scattering, cellular imaging, nuclear localization signal, cellular distinction, surface-enhanced raman, sers, biomedical sers
2463-2470
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Kuan, Wei-Li
f32d8025-2376-4408-a285-3f3d9fc11bdc
Barker, Roger
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Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
2013
Huefner, Anna
135d7fc3-aff0-4135-b98b-2c070e795ac0
Kuan, Wei-Li
f32d8025-2376-4408-a285-3f3d9fc11bdc
Barker, Roger
46d636f3-43ce-4591-970f-b897422965d9
Mahajan, Sumeet
b131f40a-479e-4432-b662-19d60d4069e9
Huefner, Anna, Kuan, Wei-Li, Barker, Roger and Mahajan, Sumeet
(2013)
Intracellular SERS nanoprobes for distinction of different neuronal cell types.
Nano Letters, 13 (6), .
(doi:10.1021/nl400448n).
Abstract
Distinction between closely related and morphologically similar cells is difficult by conventional methods especially without labelling. Using nuclear targeted gold nanoparticles as intracellular probes we demonstrate the ability to distinguish between progenitor and differentiated cell types in a human neuroblastoma cell line using surface-enhanced Raman spectroscopy (SERS). SERS spectra from the whole cell area as well as only the nucleus were analysed using principal component analysis which allowed unambiguous distinction of the different cell types. SERS spectra from the nuclear region showed the developments during cellular differentiation by identifying an increase in DNA/RNA ratio and proteins transcribed. Our approach using nuclear targeted AuNPs and SERS imaging provides label-free and non-invasive characterization which can play a vital role in identifying cells types in bio-medical stem cell research.
Text
nl400448n
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More information
e-pub ahead of print date: 2 May 2013
Published date: 2013
Additional Information:
Funded by EPSRC: Plasmon-enhanced spectroscopy and imaging inside cells (EP/H028757/1)
Keywords:
gold nanoparticles, surface-enhanced raman scattering, cellular imaging, nuclear localization signal, cellular distinction, surface-enhanced raman, sers, biomedical sers
Organisations:
Institute for Life Sciences, Organic Chemistry: SCF
Identifiers
Local EPrints ID: 352168
URI: http://eprints.soton.ac.uk/id/eprint/352168
ISSN: 1530-6984
PURE UUID: e5b71d3e-ba38-47e6-889c-fdf3d5e0aa0b
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Date deposited: 07 May 2013 13:06
Last modified: 15 Mar 2024 03:28
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Contributors
Author:
Anna Huefner
Author:
Wei-Li Kuan
Author:
Roger Barker
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