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Positional dependence of particles in microfludic impedance cytometry

Positional dependence of particles in microfludic impedance cytometry
Positional dependence of particles in microfludic impedance cytometry
Single cell impedance cytometry is a label-free electrical analysis method that requires minimal sample preparation and has been used to count and discriminate cells on the basis of their impedance properties. This paper shows experimental and numerically simulated impedance signals for test particles (6 ?m diameter polystyrene) flowing through a microfluidic channel. The variation of impedance signal with particle position is mapped using numerical simulation and these results match closely with experimental data. We demonstrate that for a nominal 40 ?m × 40 ?m channel, the impedance signal is independent of position over the majority of the channel area, but shows large experimentally verifiable variation at extreme positions. The parabolic flow profile in the channel ensures that most of the sample flows through the area of uniform signal. At high flow rates inertial focusing is observed; the particles flow in equal numbers through two equilibrium positions reducing the coefficient of variance (CV) in the impedance signals to negligible values.
1473-0197
1234-1239
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Spencer, Daniel and Morgan, Hywel (2011) Positional dependence of particles in microfludic impedance cytometry. Lab on a Chip, 11, 1234-1239.

Record type: Article

Abstract

Single cell impedance cytometry is a label-free electrical analysis method that requires minimal sample preparation and has been used to count and discriminate cells on the basis of their impedance properties. This paper shows experimental and numerically simulated impedance signals for test particles (6 ?m diameter polystyrene) flowing through a microfluidic channel. The variation of impedance signal with particle position is mapped using numerical simulation and these results match closely with experimental data. We demonstrate that for a nominal 40 ?m × 40 ?m channel, the impedance signal is independent of position over the majority of the channel area, but shows large experimentally verifiable variation at extreme positions. The parabolic flow profile in the channel ensures that most of the sample flows through the area of uniform signal. At high flow rates inertial focusing is observed; the particles flow in equal numbers through two equilibrium positions reducing the coefficient of variance (CV) in the impedance signals to negligible values.

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More information

Published date: 28 February 2011
Organisations: Electronics & Computer Science

Identifiers

Local EPrints ID: 352270
URI: https://eprints.soton.ac.uk/id/eprint/352270
ISSN: 1473-0197
PURE UUID: 8d5bacbe-8f69-4b1e-8c38-6e320d3ec462
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 08 May 2013 11:30
Last modified: 06 Jun 2018 12:45

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