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Observer agreement comparing the use of virtual slides with glass slides in the pathology review component of the POSH breast cancer cohort study

Observer agreement comparing the use of virtual slides with glass slides in the pathology review component of the POSH breast cancer cohort study
Observer agreement comparing the use of virtual slides with glass slides in the pathology review component of the POSH breast cancer cohort study
Aims: (1) To compare the use of scanned virtual slide images (virtual microscopy) with glass slides (conventional microscopy) in the assessment of morphological characteristics of breast cancers within the setting of the Prospective study of Outcomes in Sporadic versus Hereditary breast cancer (POSH), involving a cohort of women under 40 years of age, presenting with breast cancer. (2) To assess the acceptability to histopathologists of the use of virtual slide images.

Methods: 13 histopathologists from the UK and Australia participated in the POSH pathology review. The observers were asked to assess multiple morphological features such as tumour grade and type. Comparisons were made for a single observer using both virtual images and glass slides. Intra- and inter-observer variability was calculated using the ? statistic and a comparison was made between the use of each image modality.

Results: Diagnostic performance with virtual slides was comparable to conventional microscopic assessment, with the measurement of agreement best for vascular invasion, necrosis and the presence of a central scar (?=0.37-0.78), and poor for more subjective parameters such as pleomorphism, stroma, the nature of the tumour border and the degree of lymphocytic infiltrate (?=0.1).

Conclusion: Virtual slides represent an acceptable methodology for central review of breast cancer histopathology and can circumvent the need for either travel to view material, or the potential problems of sending it by post.
0021-9746
403-408
Shaw, Emily C.
6046be23-d6b4-45a1-9689-f0520b65843a
Hanby, Andrew M.
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Wheeler, Kevin
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Shaaban, Abeer M.
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Poller, David
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Barton, Sheila
4f674382-ca0b-44ad-9670-e71a0b134ef0
Treanor, Darren
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Fulford, Laura
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Walker, Rosemary A.
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Ryan, Deirdre
b7c08e42-f17a-46bd-be2d-190e155e0db3
Lakhani, Sunil R.
cc43b574-e4be-476f-b1f5-bb97f9658ada
Wells, Clive A.
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Roche, Heather
c14e4654-6427-4a99-8230-70c254bc79ce
Theaker, Jeffrey M.
a682fb66-7c35-4ae8-80bb-2425d8c43a41
Ellis, Ian O.
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Jones, J. Louise
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Eccles, D. M.
5b59bc73-11c9-4cf0-a9d5-7a8e523eee23
Shaw, Emily C.
6046be23-d6b4-45a1-9689-f0520b65843a
Hanby, Andrew M.
a7f727f8-1f6f-45d2-a536-4008c9897c0c
Wheeler, Kevin
0ac65193-3c6f-44f0-b1e0-ad503e0c241d
Shaaban, Abeer M.
8c0e9d23-e705-453e-bb5e-f34c4a303145
Poller, David
25853959-8abc-4e5d-8f9c-1bb02012a11c
Barton, Sheila
4f674382-ca0b-44ad-9670-e71a0b134ef0
Treanor, Darren
7b623a2b-a8ef-46d4-b42f-cda1fc68c697
Fulford, Laura
5f0e80c2-54cb-4a2f-8399-92740bfbd6bb
Walker, Rosemary A.
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Ryan, Deirdre
b7c08e42-f17a-46bd-be2d-190e155e0db3
Lakhani, Sunil R.
cc43b574-e4be-476f-b1f5-bb97f9658ada
Wells, Clive A.
14345304-9ab9-4fc9-b5c7-90a76bf16b95
Roche, Heather
c14e4654-6427-4a99-8230-70c254bc79ce
Theaker, Jeffrey M.
a682fb66-7c35-4ae8-80bb-2425d8c43a41
Ellis, Ian O.
83736f0c-e8e8-42c6-be2a-3597956f832a
Jones, J. Louise
a6a6c58f-c843-42c7-8832-7c970959610c
Eccles, D. M.
5b59bc73-11c9-4cf0-a9d5-7a8e523eee23

Shaw, Emily C., Hanby, Andrew M., Wheeler, Kevin, Shaaban, Abeer M., Poller, David, Barton, Sheila, Treanor, Darren, Fulford, Laura, Walker, Rosemary A., Ryan, Deirdre, Lakhani, Sunil R., Wells, Clive A., Roche, Heather, Theaker, Jeffrey M., Ellis, Ian O., Jones, J. Louise and Eccles, D. M. (2012) Observer agreement comparing the use of virtual slides with glass slides in the pathology review component of the POSH breast cancer cohort study. Journal of Clinical Pathology, 65 (5), 403-408. (doi:10.1136/jclinpath-2011-200369). (PMID:22447915)

Record type: Article

Abstract

Aims: (1) To compare the use of scanned virtual slide images (virtual microscopy) with glass slides (conventional microscopy) in the assessment of morphological characteristics of breast cancers within the setting of the Prospective study of Outcomes in Sporadic versus Hereditary breast cancer (POSH), involving a cohort of women under 40 years of age, presenting with breast cancer. (2) To assess the acceptability to histopathologists of the use of virtual slide images.

Methods: 13 histopathologists from the UK and Australia participated in the POSH pathology review. The observers were asked to assess multiple morphological features such as tumour grade and type. Comparisons were made for a single observer using both virtual images and glass slides. Intra- and inter-observer variability was calculated using the ? statistic and a comparison was made between the use of each image modality.

Results: Diagnostic performance with virtual slides was comparable to conventional microscopic assessment, with the measurement of agreement best for vascular invasion, necrosis and the presence of a central scar (?=0.37-0.78), and poor for more subjective parameters such as pleomorphism, stroma, the nature of the tumour border and the degree of lymphocytic infiltrate (?=0.1).

Conclusion: Virtual slides represent an acceptable methodology for central review of breast cancer histopathology and can circumvent the need for either travel to view material, or the potential problems of sending it by post.

Full text not available from this repository.

More information

e-pub ahead of print date: 23 March 2012
Published date: May 2012
Organisations: Cancer Sciences, Human Development & Health, Clinical Trials Unit

Identifiers

Local EPrints ID: 352329
URI: https://eprints.soton.ac.uk/id/eprint/352329
ISSN: 0021-9746
PURE UUID: c8a410d6-b247-47ee-91be-1ccf80bcef15
ORCID for Sheila Barton: ORCID iD orcid.org/0000-0003-4963-4242

Catalogue record

Date deposited: 10 May 2013 11:51
Last modified: 07 Aug 2019 00:45

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Contributors

Author: Emily C. Shaw
Author: Andrew M. Hanby
Author: Kevin Wheeler
Author: Abeer M. Shaaban
Author: David Poller
Author: Sheila Barton ORCID iD
Author: Darren Treanor
Author: Laura Fulford
Author: Rosemary A. Walker
Author: Deirdre Ryan
Author: Sunil R. Lakhani
Author: Clive A. Wells
Author: Heather Roche
Author: Jeffrey M. Theaker
Author: Ian O. Ellis
Author: J. Louise Jones
Author: D. M. Eccles

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