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A sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus

A sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus
A sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus
The 3'-terminal labelling procedure for analyzing the genome segment profile of field isolates of rotavirus (Clarke and McCrae, 1981, J. Virol. Methods 2, 203) has been further developed to produce a rapid and sensitive method for generating diagnostic fingerprints from individual species of double-stranded RNA. The fingerprints were obtained by a one-dimensional resolution of overlapping terminally labelled oligonucleotides produced by partial nuclease digestion with a base-specific nuclease. This fingerprinting method should be of great value in characterizing the molecular details of genome segment variation and will facilitate detailed molecular epidemiological studies of this important virus group.
0166-0934
261-9
Clarke, I. N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, M. A.
918087fb-1585-458f-81ac-43e33fd7e3e2
Clarke, I. N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, M. A.
918087fb-1585-458f-81ac-43e33fd7e3e2

Clarke, I. N. and McCrae, M. A. (1981) A sensitive method for the production of diagnostic fingerprints of the genome segments of field isolates of rotavirus. Journal of Virological Methods, 3 (5), 261-9. (PMID:6277973)

Record type: Article

Abstract

The 3'-terminal labelling procedure for analyzing the genome segment profile of field isolates of rotavirus (Clarke and McCrae, 1981, J. Virol. Methods 2, 203) has been further developed to produce a rapid and sensitive method for generating diagnostic fingerprints from individual species of double-stranded RNA. The fingerprints were obtained by a one-dimensional resolution of overlapping terminally labelled oligonucleotides produced by partial nuclease digestion with a base-specific nuclease. This fingerprinting method should be of great value in characterizing the molecular details of genome segment variation and will facilitate detailed molecular epidemiological studies of this important virus group.

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More information

Published date: December 1981
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352616
URI: https://eprints.soton.ac.uk/id/eprint/352616
ISSN: 0166-0934
PURE UUID: 94f08ba9-2a03-4738-957a-1f6f901f4b86
ORCID for I. N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 21 May 2013 09:42
Last modified: 06 Jun 2018 13:19

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Contributors

Author: I. N. Clarke ORCID iD
Author: M. A. McCrae

University divisions

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