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A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus

A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus
A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus
The direct extraction of double-stranded (ds) RNA from virus-infected faeces has provided a method for analysing a large number of field isolates of rotavirus simultaneously. 3' terminal labelling of the extracted RNA with [32P]Cp using T4 RNA ligase has been employed to give a very sensitive and reproducible method for visualising the rotavirus genome RNA proflies.
0166-0934
203-209
Clarke, I. N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, M. A.
918087fb-1585-458f-81ac-43e33fd7e3e2
Clarke, I. N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, M. A.
918087fb-1585-458f-81ac-43e33fd7e3e2

Clarke, I. N. and McCrae, M. A. (1981) A rapid and sensitive method for analysing the genome profiles of field isolates of rotavirus. Journal of Virological Methods, 2 (4), 203-209. (PMID:6262345)

Record type: Article

Abstract

The direct extraction of double-stranded (ds) RNA from virus-infected faeces has provided a method for analysing a large number of field isolates of rotavirus simultaneously. 3' terminal labelling of the extracted RNA with [32P]Cp using T4 RNA ligase has been employed to give a very sensitive and reproducible method for visualising the rotavirus genome RNA proflies.

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More information

Published date: March 1981
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352617
URI: https://eprints.soton.ac.uk/id/eprint/352617
ISSN: 0166-0934
PURE UUID: 14aaa82d-0316-462e-887d-26acb6a20b19
ORCID for I. N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 21 May 2013 09:02
Last modified: 06 Jun 2018 13:19

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Contributors

Author: I. N. Clarke ORCID iD
Author: M. A. McCrae

University divisions

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