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Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates

Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates
Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates
Detailed structural studies were undertaken on five isolates of bovine rotavirus which showed variability in the migration patterns of their genome segments on electrophoresis in polyacrylamide gels. The individual genome segments of each isolate were characterized by partial digestion of terminally radiolabeled RNA with a base-specific nuclease. This analysis showed that whereas mobility variations were always associated with detectable changes in nucleotide sequence, sequence changes at least as great as those found in segments showing electrophoretic mobility variations were also detected in segments showing no mobility variation. Evidence for the occurrence of genome segment reassortment between viruses in the field was obtained from analyzing the species 11 RNAs from these five isolates. The overall conclusion from these results is that great care is required in the interpretation of simple genome profile analysis of different isolates for epidemiological purposes and that classification of these viruses solely on the basis of genome electropherotype could be misleading.
0019-9567
492-497
Clarke, I. N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, M. A.
918087fb-1585-458f-81ac-43e33fd7e3e2
Clarke, I. N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, M. A.
918087fb-1585-458f-81ac-43e33fd7e3e2

Clarke, I. N. and McCrae, M. A. (1982) Structural analysis of electrophoretic variation in the genome profiles of rotavirus field isolates. Infection and Immunity, 36 (2), 492-497. (PMID:225545)

Record type: Article

Abstract

Detailed structural studies were undertaken on five isolates of bovine rotavirus which showed variability in the migration patterns of their genome segments on electrophoresis in polyacrylamide gels. The individual genome segments of each isolate were characterized by partial digestion of terminally radiolabeled RNA with a base-specific nuclease. This analysis showed that whereas mobility variations were always associated with detectable changes in nucleotide sequence, sequence changes at least as great as those found in segments showing electrophoretic mobility variations were also detected in segments showing no mobility variation. Evidence for the occurrence of genome segment reassortment between viruses in the field was obtained from analyzing the species 11 RNAs from these five isolates. The overall conclusion from these results is that great care is required in the interpretation of simple genome profile analysis of different isolates for epidemiological purposes and that classification of these viruses solely on the basis of genome electropherotype could be misleading.

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Published date: May 1982
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352618
URI: https://eprints.soton.ac.uk/id/eprint/352618
ISSN: 0019-9567
PURE UUID: 94667396-f2c8-4763-b0c7-4a3179a4514a
ORCID for I. N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 22 May 2013 11:33
Last modified: 06 Jun 2018 13:19

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Contributors

Author: I. N. Clarke ORCID iD
Author: M. A. McCrae

University divisions

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