The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses
The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses
The use of T1 RNase fingerprinting of terminally labelled genomic double-stranded RNA species from various rotavirus isolates, to analyse the near terminal G-residue positions, has revealed an RNA species-specific fingerprint pattern covering approximately 40 nucleotides at the termini. These RNA species-specific terminal fingerprint patterns were found to be conserved in both rotavirus RNAs isolated from various animal species, and in isolates from a single animal species where gross divergence of internal RNA sequence for a particular RNA species was evident. This conservation of near terminal G-residue positions suggests that, internal to the short regions of absolute terminal sequence conservation that we have previously shown to be present on all rotavirus RNA species, there is a region of conserved sequence which is specific for a particular RNA species.
1877-1884
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, Malcolm A.
f99d4d28-2d4a-4f6a-859f-cebe5df27a94
September 1983
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, Malcolm A.
f99d4d28-2d4a-4f6a-859f-cebe5df27a94
Clarke, Ian N. and McCrae, Malcolm A.
(1983)
The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses.
Journal of General Virology, 64 (9), .
(PMID:6310030)
Abstract
The use of T1 RNase fingerprinting of terminally labelled genomic double-stranded RNA species from various rotavirus isolates, to analyse the near terminal G-residue positions, has revealed an RNA species-specific fingerprint pattern covering approximately 40 nucleotides at the termini. These RNA species-specific terminal fingerprint patterns were found to be conserved in both rotavirus RNAs isolated from various animal species, and in isolates from a single animal species where gross divergence of internal RNA sequence for a particular RNA species was evident. This conservation of near terminal G-residue positions suggests that, internal to the short regions of absolute terminal sequence conservation that we have previously shown to be present on all rotavirus RNA species, there is a region of conserved sequence which is specific for a particular RNA species.
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Published date: September 1983
Organisations:
Faculty of Medicine
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Local EPrints ID: 352620
URI: http://eprints.soton.ac.uk/id/eprint/352620
ISSN: 0022-1317
PURE UUID: 83c04182-61ac-4f5b-8725-8f80ee3499e1
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Date deposited: 23 May 2013 15:05
Last modified: 11 Dec 2021 02:35
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Author:
Malcolm A. McCrae
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