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The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses

The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses
The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses
The use of T1 RNase fingerprinting of terminally labelled genomic double-stranded RNA species from various rotavirus isolates, to analyse the near terminal G-residue positions, has revealed an RNA species-specific fingerprint pattern covering approximately 40 nucleotides at the termini. These RNA species-specific terminal fingerprint patterns were found to be conserved in both rotavirus RNAs isolated from various animal species, and in isolates from a single animal species where gross divergence of internal RNA sequence for a particular RNA species was evident. This conservation of near terminal G-residue positions suggests that, internal to the short regions of absolute terminal sequence conservation that we have previously shown to be present on all rotavirus RNA species, there is a region of conserved sequence which is specific for a particular RNA species.
0022-1317
1877-1884
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, Malcolm A.
f99d4d28-2d4a-4f6a-859f-cebe5df27a94
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
McCrae, Malcolm A.
f99d4d28-2d4a-4f6a-859f-cebe5df27a94

Clarke, Ian N. and McCrae, Malcolm A. (1983) The molecular biology of rotaviruses. VI. RNA species-specific terminal conservation in rotaviruses. Journal of General Virology, 64 (9), 1877-1884. (PMID:6310030)

Record type: Article

Abstract

The use of T1 RNase fingerprinting of terminally labelled genomic double-stranded RNA species from various rotavirus isolates, to analyse the near terminal G-residue positions, has revealed an RNA species-specific fingerprint pattern covering approximately 40 nucleotides at the termini. These RNA species-specific terminal fingerprint patterns were found to be conserved in both rotavirus RNAs isolated from various animal species, and in isolates from a single animal species where gross divergence of internal RNA sequence for a particular RNA species was evident. This conservation of near terminal G-residue positions suggests that, internal to the short regions of absolute terminal sequence conservation that we have previously shown to be present on all rotavirus RNA species, there is a region of conserved sequence which is specific for a particular RNA species.

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Published date: September 1983
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352620
URI: https://eprints.soton.ac.uk/id/eprint/352620
ISSN: 0022-1317
PURE UUID: 83c04182-61ac-4f5b-8725-8f80ee3499e1
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 23 May 2013 15:05
Last modified: 06 Jun 2018 13:19

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Contributors

Author: Ian N. Clarke ORCID iD
Author: Malcolm A. McCrae

University divisions

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