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Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.

Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.
Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis.
Two overlapping genomic fragments have been cloned from Chlamydia trachomatis serovar L1 DNA. Sequence determination of 2530 bp has revealed two open reading frames coding for 'cysteine-rich' (Cr) proteins. One of these proteins was confirmed, by analysis of the inferred amino acid sequence, as the 60-kDa Cr outer membrane protein associated with differentiation of reticulate bodies (RBs) into elementary bodies (EBs). The other smaller 15-kDa protein contained a high percentage of methionine and cysteine and may correspond to a reported smaller and co-ordinately synthesised Cr outer-membrane protein also associated with RB to EB differentiation. Sequencing showed three potential stem-loop structures within the 5', 3' and intergenic regions of the cloned fragment. Southern-blot analysis revealed that the cloned fragment is conserved in ten serovars of C. trachomatis and that a strongly cross-hybridising fragment is also present in Chlamydia psittaci.
0378-1119
307-314
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
80edb68e-7ce4-4724-9737-f078f8ffbb70
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
80edb68e-7ce4-4724-9737-f078f8ffbb70
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77

Clarke, I.N., Ward, M.E. and Lambden, P.R. (1988) Molecular cloning and sequence analysis of a developmentally regulated cysteine-rich outer membrane protein from Chlamydia trachomatis. Gene, 71 (2), 307-314. (PMID:3066701)

Record type: Article

Abstract

Two overlapping genomic fragments have been cloned from Chlamydia trachomatis serovar L1 DNA. Sequence determination of 2530 bp has revealed two open reading frames coding for 'cysteine-rich' (Cr) proteins. One of these proteins was confirmed, by analysis of the inferred amino acid sequence, as the 60-kDa Cr outer membrane protein associated with differentiation of reticulate bodies (RBs) into elementary bodies (EBs). The other smaller 15-kDa protein contained a high percentage of methionine and cysteine and may correspond to a reported smaller and co-ordinately synthesised Cr outer-membrane protein also associated with RB to EB differentiation. Sequencing showed three potential stem-loop structures within the 5', 3' and intergenic regions of the cloned fragment. Southern-blot analysis revealed that the cloned fragment is conserved in ten serovars of C. trachomatis and that a strongly cross-hybridising fragment is also present in Chlamydia psittaci.

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Published date: 30 November 1988
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352622
URI: https://eprints.soton.ac.uk/id/eprint/352622
ISSN: 0378-1119
PURE UUID: d89f681c-b7e8-4d4c-977c-c626bcfa3570
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 03 Jun 2013 14:29
Last modified: 06 Jun 2018 13:19

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Contributors

Author: I.N. Clarke ORCID iD
Author: M.E. Ward
Author: P.R. Lambden

University divisions

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