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High-level expression and epitope localization of the major outer membrane protein of Chlamydia trachomatis serovar L1

High-level expression and epitope localization of the major outer membrane protein of Chlamydia trachomatis serovar L1
High-level expression and epitope localization of the major outer membrane protein of Chlamydia trachomatis serovar L1
Fragments of the gene encoding the major outer membrane porin protein (MOMP) of Chlamydia trachomatis serovar L1 were ligated into the pUC plasmid vectors to give a series of overlapping recombinants expressing MOMP from the lac promoter. Induction of this promoter with IPTG leads to high-level expression of the recombinant porin protein. Electron microscopy shows the presence of insoluble inclusions within the Escherichia coli host cells. Probing the expressed MOMP fragments with a set of monoclonal antibodies permitted localization of the four binding sites (epitopes) of primary-sequence-dependent monoclonal antibodies that exhibit genus-, species-, subspecies- and type (serovar)-specific reactivities.
0950-382X
681-685
Pickett, M.A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Pickett, M.A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Pickett, M.A., Ward, M.E. and Clarke, I.N. (1988) High-level expression and epitope localization of the major outer membrane protein of Chlamydia trachomatis serovar L1. Molecular Microbiology, 2 (5), 681-685. (PMID:2460720)

Record type: Article

Abstract

Fragments of the gene encoding the major outer membrane porin protein (MOMP) of Chlamydia trachomatis serovar L1 were ligated into the pUC plasmid vectors to give a series of overlapping recombinants expressing MOMP from the lac promoter. Induction of this promoter with IPTG leads to high-level expression of the recombinant porin protein. Electron microscopy shows the presence of insoluble inclusions within the Escherichia coli host cells. Probing the expressed MOMP fragments with a set of monoclonal antibodies permitted localization of the four binding sites (epitopes) of primary-sequence-dependent monoclonal antibodies that exhibit genus-, species-, subspecies- and type (serovar)-specific reactivities.

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Published date: September 1988
Organisations: Faculty of Medicine

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Local EPrints ID: 352623
URI: https://eprints.soton.ac.uk/id/eprint/352623
ISSN: 0950-382X
PURE UUID: d6c7e47b-00f1-404f-b453-e4a72e63cd36
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 23 May 2013 14:38
Last modified: 06 Jun 2018 13:19

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Contributors

Author: M.A. Pickett
Author: M.E. Ward
Author: I.N. Clarke ORCID iD

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