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Chlamydia trachomatis 60 kDa cysteine rich outer membrane protein: sequence homology between trachoma and LGV biovars

Chlamydia trachomatis 60 kDa cysteine rich outer membrane protein: sequence homology between trachoma and LGV biovars
Chlamydia trachomatis 60 kDa cysteine rich outer membrane protein: sequence homology between trachoma and LGV biovars
An 820 bp AccI-PstI fragment of the 60 kDa cysteine rich outer membrane protein (CrP) gene from C. trachomatis serovar L1 was used as a probe to locate the 60 kDa CrP gene of a recent serovar B trachoma isolate (Jali 20/OT). The probe hybridized to a single 1.8 kb SpeI fragment in Southern blot analyses of different restriction endonuclease digests of C. trachomatis serovar B DNA. This fragment was ligated into Lambda Zap II arms and Bluescript SK(-) recombinants, released by infection with the helper phage R408, were used as template for DNA sequence determination. Sequence analysis demonstrated a very high level of homology between the Jali 20/OT 60 kDa CrP and the previously published serovar L1 60 kDa CrP with only 8 out of 507 amino acid substitutions between the two proteins.
0378-1097
293-297
Watson, M.W.
51934130-1422-4ca9-8a54-a0903f943519
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Ward, M.E.
80edb68e-7ce4-4724-9737-f078f8ffbb70
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Watson, M.W.
51934130-1422-4ca9-8a54-a0903f943519
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Ward, M.E.
80edb68e-7ce4-4724-9737-f078f8ffbb70
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Watson, M.W., Lambden, P.R., Ward, M.E. and Clarke, I.N. (1989) Chlamydia trachomatis 60 kDa cysteine rich outer membrane protein: sequence homology between trachoma and LGV biovars. FEMS Microbiology Letters, 65 (3), 293-297. (doi:10.1111/j.1574-6968.1989.tb03676.x). (PMID:2612891)

Record type: Article

Abstract

An 820 bp AccI-PstI fragment of the 60 kDa cysteine rich outer membrane protein (CrP) gene from C. trachomatis serovar L1 was used as a probe to locate the 60 kDa CrP gene of a recent serovar B trachoma isolate (Jali 20/OT). The probe hybridized to a single 1.8 kb SpeI fragment in Southern blot analyses of different restriction endonuclease digests of C. trachomatis serovar B DNA. This fragment was ligated into Lambda Zap II arms and Bluescript SK(-) recombinants, released by infection with the helper phage R408, were used as template for DNA sequence determination. Sequence analysis demonstrated a very high level of homology between the Jali 20/OT 60 kDa CrP and the previously published serovar L1 60 kDa CrP with only 8 out of 507 amino acid substitutions between the two proteins.

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Published date: December 1989
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352625
URI: http://eprints.soton.ac.uk/id/eprint/352625
ISSN: 0378-1097
PURE UUID: 31b96c35-8752-4a8f-94db-5ddb4f60b893
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 03 Jun 2013 15:21
Last modified: 18 Feb 2021 16:32

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Contributors

Author: M.W. Watson
Author: P.R. Lambden
Author: M.E. Ward
Author: I.N. Clarke ORCID iD

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