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Surface-exposed epitopes on the major outer-membrane protein of Chlamydia trachomatis defined with peptide antisera

Surface-exposed epitopes on the major outer-membrane protein of Chlamydia trachomatis defined with peptide antisera
Surface-exposed epitopes on the major outer-membrane protein of Chlamydia trachomatis defined with peptide antisera
The surface exposure of computer-predicted, linear B-cell epitopes on the major outer-membrane protein (MOMP) of Chlamydia trachomatis serovar B was assessed using antibodies raised against synthetic peptides in conjunction with immunogold transmission electron microscopy. Several of the chosen peptides elicited antibodies which reacted with both denatured and native MOMP. The majority of the exposed epitopes were found within the variable segments of MOMP. For each of the epitopes identified, the extent of their surface accessibility varied both among individual organisms and different developmental forms. Evidence for two distinct subspecies-specific epitopes within VS4 is presented.
0022-1287
3219-3228
Conlan, J.W.
80eceadc-14b1-481d-9812-14e7562d332f
Ferris, S.
3b0a8d88-429b-4379-8872-7d5ade3ffd54
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468
Conlan, J.W.
80eceadc-14b1-481d-9812-14e7562d332f
Ferris, S.
3b0a8d88-429b-4379-8872-7d5ade3ffd54
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468

Conlan, J.W., Ferris, S., Clarke, I.N. and Ward, M.E. (1989) Surface-exposed epitopes on the major outer-membrane protein of Chlamydia trachomatis defined with peptide antisera. Journal of General Microbiology, 135 (12), 3219-3228. (PMID:2483992)

Record type: Article

Abstract

The surface exposure of computer-predicted, linear B-cell epitopes on the major outer-membrane protein (MOMP) of Chlamydia trachomatis serovar B was assessed using antibodies raised against synthetic peptides in conjunction with immunogold transmission electron microscopy. Several of the chosen peptides elicited antibodies which reacted with both denatured and native MOMP. The majority of the exposed epitopes were found within the variable segments of MOMP. For each of the epitopes identified, the extent of their surface accessibility varied both among individual organisms and different developmental forms. Evidence for two distinct subspecies-specific epitopes within VS4 is presented.

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Published date: December 1989
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352626
URI: http://eprints.soton.ac.uk/id/eprint/352626
ISSN: 0022-1287
PURE UUID: 1e4cdf2e-eaa9-4596-b01d-a4e1f3ccc002
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 03 Jun 2013 15:26
Last modified: 18 Feb 2021 16:32

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Contributors

Author: J.W. Conlan
Author: S. Ferris
Author: I.N. Clarke ORCID iD
Author: M.E. Ward

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