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Isolation of recombinant fragments of the major outer-membrane protein of Chlamydia trachomatis: their potential as subunit vaccines

Isolation of recombinant fragments of the major outer-membrane protein of Chlamydia trachomatis: their potential as subunit vaccines
Isolation of recombinant fragments of the major outer-membrane protein of Chlamydia trachomatis: their potential as subunit vaccines
Recombinant fragments of the major outer-membrane protein (MOMP) of Chlamydia trachomatis, expressed at high levels in Escherichia coli, were isolated and purified. Antisera to the recombinant proteins reacted preferentially with overlapping synthetic peptides covering the immunoaccessible variable segments of MOMP. These sera also reacted in a species-specific manner with the surface of intact infectious elementary bodies, and in a Chlamydia genus-specific manner in assays using denatured or bound chlamydial antigens. The ability of recombinant MOMP preparations to elicit antibody to the surface of chlamydial elementary bodies raises the possibility that these proteins may be useful for chlamydial vaccine development.
1350-0872
2013-20
Conlan, J.W.
80eceadc-14b1-481d-9812-14e7562d332f
Ferris, S.
3b0a8d88-429b-4379-8872-7d5ade3ffd54
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468
Conlan, J.W.
80eceadc-14b1-481d-9812-14e7562d332f
Ferris, S.
3b0a8d88-429b-4379-8872-7d5ade3ffd54
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468

Conlan, J.W., Ferris, S., Clarke, I.N. and Ward, M.E. (1990) Isolation of recombinant fragments of the major outer-membrane protein of Chlamydia trachomatis: their potential as subunit vaccines. Microbiology, 136 (10), 2013-20. (PMID:1702826)

Record type: Article

Abstract

Recombinant fragments of the major outer-membrane protein (MOMP) of Chlamydia trachomatis, expressed at high levels in Escherichia coli, were isolated and purified. Antisera to the recombinant proteins reacted preferentially with overlapping synthetic peptides covering the immunoaccessible variable segments of MOMP. These sera also reacted in a species-specific manner with the surface of intact infectious elementary bodies, and in a Chlamydia genus-specific manner in assays using denatured or bound chlamydial antigens. The ability of recombinant MOMP preparations to elicit antibody to the surface of chlamydial elementary bodies raises the possibility that these proteins may be useful for chlamydial vaccine development.

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Published date: October 1990
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352630
URI: https://eprints.soton.ac.uk/id/eprint/352630
ISSN: 1350-0872
PURE UUID: 544716d2-bdee-4d2a-84cb-b2a5d39b4962
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 03 Jun 2013 15:32
Last modified: 06 Jun 2018 13:19

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Contributors

Author: J.W. Conlan
Author: S. Ferris
Author: I.N. Clarke ORCID iD
Author: M.E. Ward

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