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Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus

Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus
Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus
The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The protein sequence demonstrated low homology with the group A VP6 sequences (41.7 to 42.7%) and high homology (88.9%) with the porcine group C VP6 sequence. However, the protein sequence alignments revealed a region of 10 amino acids that were significantly different between the human and the porcine group C viruses.
0042-6822
781-785
Cooke, S.J
f3292a2f-7379-460b-81ef-b5d4fd3bc572
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Caul, E.O.
3ab60e6e-ca41-4196-a205-4c0075fd3767
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Cooke, S.J
f3292a2f-7379-460b-81ef-b5d4fd3bc572
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Caul, E.O.
3ab60e6e-ca41-4196-a205-4c0075fd3767
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Cooke, S.J, Lambden, P.R., Caul, E.O. and Clarke, I.N. (1991) Molecular cloning, sequence analysis and coding assignment of the major inner capsid protein gene of human group C rotavirus. Virology, 184 (2), 781-785. (doi:10.1016/0042-6822(91)90452-H). (PMID:1653500)

Record type: Article

Abstract

The VP6 gene of human group C rotavirus was cloned and sequenced. Hybridization to the human group C and the porcine group C/Cowden dsRNA genomes assigned this coding sequence to segment 5. The complete human VP6 sequence contained an open reading frame of 1185 nucleotides (395 amino acids; deduced Mr 44,669 Da). The protein sequence demonstrated low homology with the group A VP6 sequences (41.7 to 42.7%) and high homology (88.9%) with the porcine group C VP6 sequence. However, the protein sequence alignments revealed a region of 10 amino acids that were significantly different between the human and the porcine group C viruses.

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Published date: October 1991
Organisations: Faculty of Medicine

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Local EPrints ID: 352636
URI: http://eprints.soton.ac.uk/id/eprint/352636
ISSN: 0042-6822
PURE UUID: 94eb183a-0ec9-4dba-b9f2-587fb85f8bfc
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 04 Jun 2013 11:14
Last modified: 18 Feb 2021 16:32

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Contributors

Author: S.J Cooke
Author: P.R. Lambden
Author: E.O. Caul
Author: I.N. Clarke ORCID iD

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