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Deoxyribonucleic acid amplification and hybridisation in Crohn's disease using a chlamydial plasmid probe

Deoxyribonucleic acid amplification and hybridisation in Crohn's disease using a chlamydial plasmid probe
Deoxyribonucleic acid amplification and hybridisation in Crohn's disease using a chlamydial plasmid probe
The possibility that Crohn's disease is caused by infection with Chlamydia trachomatis was examined by probing for chlamydial plasmid deoxyribonucleic acid (DNA) in DNA extracts from Crohn's disease tissue and by means of a serological study. Gut DNA extracts were obtained from 10 patients with Crohn's disease and four control subjects and were probed with a chlamydial plasmid probe after Southern blotting. The polymerase chain reaction was also used to amplify any chlamydial plasmid DNA present in tissue DNA extracts, before Southern blotting and probing. Chlamydial proctitis control specimens were not available: gut DNA extracts mixed with traces of chlamydia plasmid served as positive controls. Using these techniques, no chlamydial plasmid DNA sequences were found in Crohn's disease tissue. An enzyme linked immunosorbent assay for C trachomatis LI was performed on 48 patients with Crohn's disease and 48 control subjects. Seropositivity was present in 14.6% of patients and 29% of control subjects and was not statistically significant (p greater than 0.05). The failure to show chlamydial DNA and the lack of serological response to chlamydia make C trachomatis infection a very unlikely factor in the pathogenesis of Crohn's disease.
0017-5749
1011-1015
McGarity, B.H.
5da8bf92-6b38-476a-91da-3e28d75b8ded
Robertson, D.A.
574e743e-a6b6-4699-93cb-24d45b4a0f74
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Wright, R.
462b5578-a979-4e5c-8fa8-a3f368a37531
McGarity, B.H.
5da8bf92-6b38-476a-91da-3e28d75b8ded
Robertson, D.A.
574e743e-a6b6-4699-93cb-24d45b4a0f74
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Wright, R.
462b5578-a979-4e5c-8fa8-a3f368a37531

McGarity, B.H., Robertson, D.A., Clarke, I.N. and Wright, R. (1991) Deoxyribonucleic acid amplification and hybridisation in Crohn's disease using a chlamydial plasmid probe. Gut, 32 (9), 1011-1015. (doi:10.1136/gut.32.9.1011). (PMID:1916481)

Record type: Article

Abstract

The possibility that Crohn's disease is caused by infection with Chlamydia trachomatis was examined by probing for chlamydial plasmid deoxyribonucleic acid (DNA) in DNA extracts from Crohn's disease tissue and by means of a serological study. Gut DNA extracts were obtained from 10 patients with Crohn's disease and four control subjects and were probed with a chlamydial plasmid probe after Southern blotting. The polymerase chain reaction was also used to amplify any chlamydial plasmid DNA present in tissue DNA extracts, before Southern blotting and probing. Chlamydial proctitis control specimens were not available: gut DNA extracts mixed with traces of chlamydia plasmid served as positive controls. Using these techniques, no chlamydial plasmid DNA sequences were found in Crohn's disease tissue. An enzyme linked immunosorbent assay for C trachomatis LI was performed on 48 patients with Crohn's disease and 48 control subjects. Seropositivity was present in 14.6% of patients and 29% of control subjects and was not statistically significant (p greater than 0.05). The failure to show chlamydial DNA and the lack of serological response to chlamydia make C trachomatis infection a very unlikely factor in the pathogenesis of Crohn's disease.

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Published date: September 1991
Organisations: Faculty of Medicine

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Local EPrints ID: 352637
URI: http://eprints.soton.ac.uk/id/eprint/352637
ISSN: 0017-5749
PURE UUID: fababa8a-b69c-4bac-a7f2-c90ffada7a97
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 04 Jun 2013 11:20
Last modified: 18 Feb 2021 16:32

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Contributors

Author: B.H. McGarity
Author: D.A. Robertson
Author: I.N. Clarke ORCID iD
Author: R. Wright

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