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Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogens

Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogens
Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogens
The major outer-membrane protein (MOMP) of Chlamydia trachomatis is the focus of attention for chlamydial vaccine design, particularly those serovar- and subspecies-specific epitopes which provoke neutralizing immune responses. Selected surface-exposed B-cell epitopes of MOMP, incorporating B-subspecies specificities, were expressed as fusions with LamB, an inducible outer-membrane transport protein of Escherichia coli. These recombinant chlamydial-LamB proteins were correctly transported to the outer membrane of both E. coli and an aro A mutant of Salmonella typhimurium. The immunogenicity of the constructs was investigated in a mouse model of chlamydial salpingitis. After oral immunization, recombinant S. typhimurium were recovered from the livers of mice for up to two weeks, and a serum IgG response was induced both to the Salmonella and to the inserted chlamydial epitopes. By contrast, intravenous inoculation was ineffective. Although these LamB fusions proved only weakly immunogenic, this approach should be useful for investigating the ability of attenuated S. typhimurium vaccines incorporating chlamydial epitopes to stimulate protective mucosal immunity in the mouse model of chlamydial salpingitis.
1350-0872
1557-1564
Hayes, L.J.
1bbf605d-315f-4099-beeb-f9ac2018e608
Conlan, J.W.
80eceadc-14b1-481d-9812-14e7562d332f
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Hayes, L.J.
1bbf605d-315f-4099-beeb-f9ac2018e608
Conlan, J.W.
80eceadc-14b1-481d-9812-14e7562d332f
Everson, J.S.
8f5e2cbc-b8f9-4ba6-9140-d726764d6c14
Ward, M.E.
a199bc96-75b6-415c-bffe-e68e8a00b468
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Hayes, L.J., Conlan, J.W., Everson, J.S., Ward, M.E. and Clarke, I.N. (1991) Chlamydia trachomatis major outer membrane protein epitopes expressed as fusions with LamB in an attenuated aroA strain of Salmonella typhimurium; their application as potential immunogens. Microbiology, 137 (7), 1557-1564. (doi:10.1099/00221287-137-7-1557). (PMID:1720166)

Record type: Article

Abstract

The major outer-membrane protein (MOMP) of Chlamydia trachomatis is the focus of attention for chlamydial vaccine design, particularly those serovar- and subspecies-specific epitopes which provoke neutralizing immune responses. Selected surface-exposed B-cell epitopes of MOMP, incorporating B-subspecies specificities, were expressed as fusions with LamB, an inducible outer-membrane transport protein of Escherichia coli. These recombinant chlamydial-LamB proteins were correctly transported to the outer membrane of both E. coli and an aro A mutant of Salmonella typhimurium. The immunogenicity of the constructs was investigated in a mouse model of chlamydial salpingitis. After oral immunization, recombinant S. typhimurium were recovered from the livers of mice for up to two weeks, and a serum IgG response was induced both to the Salmonella and to the inserted chlamydial epitopes. By contrast, intravenous inoculation was ineffective. Although these LamB fusions proved only weakly immunogenic, this approach should be useful for investigating the ability of attenuated S. typhimurium vaccines incorporating chlamydial epitopes to stimulate protective mucosal immunity in the mouse model of chlamydial salpingitis.

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Published date: July 1991
Organisations: Faculty of Medicine

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Local EPrints ID: 352638
URI: http://eprints.soton.ac.uk/id/eprint/352638
ISSN: 1350-0872
PURE UUID: c93a614f-db46-4ddc-a53a-c99e5e241f0e
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 04 Jun 2013 11:24
Last modified: 18 Feb 2021 16:32

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Contributors

Author: L.J. Hayes
Author: J.W. Conlan
Author: J.S. Everson
Author: M.E. Ward
Author: I.N. Clarke ORCID iD

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