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Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene

Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene
Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene
The 60-kDa cysteine-rich outer membrane protein genes of Chlamydia psittaci, Chlamydia pneumoniae, and Chlamydia trachomatis have very different 5' ends, but two areas flanking this variable region show absolute sequence conservation. This observation permitted differentiation of the three species of Chlamydia by the polymerase chain reaction (PCR), forming the basis of a diagnostic test for chlamydial infections. The PCR product containing the variable region of the respective 60-kDa CrP genes was also subjected to restriction endonuclease digestion, enabling differentiation of individual type strains of C. psittaci. Differentiation was possible between lymphogranuloma venereum and trachoma isolates of C. trachomatis. The PCR-based diagnostic test was successful with all strains of chlamydiae studied. The PCR primers showed high specificity and did not produce any product with common bacterial pathogens that may share the same sites of infection.
0095-1137
1188-1193
Watson, M.W.
51934130-1422-4ca9-8a54-a0903f943519
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Watson, M.W.
51934130-1422-4ca9-8a54-a0903f943519
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Watson, M.W., Lambden, P.R. and Clarke, I.N. (1991) Genetic diversity and identification of human infection by amplification of the chlamydial 60-kilodalton cysteine-rich outer membrane protein gene. Journal of Clinical Microbiology, 29 (6), 1188-1193. (PMID:1864938)

Record type: Article

Abstract

The 60-kDa cysteine-rich outer membrane protein genes of Chlamydia psittaci, Chlamydia pneumoniae, and Chlamydia trachomatis have very different 5' ends, but two areas flanking this variable region show absolute sequence conservation. This observation permitted differentiation of the three species of Chlamydia by the polymerase chain reaction (PCR), forming the basis of a diagnostic test for chlamydial infections. The PCR product containing the variable region of the respective 60-kDa CrP genes was also subjected to restriction endonuclease digestion, enabling differentiation of individual type strains of C. psittaci. Differentiation was possible between lymphogranuloma venereum and trachoma isolates of C. trachomatis. The PCR-based diagnostic test was successful with all strains of chlamydiae studied. The PCR primers showed high specificity and did not produce any product with common bacterial pathogens that may share the same sites of infection.

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Published date: June 1991
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352639
URI: http://eprints.soton.ac.uk/id/eprint/352639
ISSN: 0095-1137
PURE UUID: e8ae4d53-e041-45bd-9e7c-b6fd2d264481
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 04 Jun 2013 11:29
Last modified: 18 Feb 2021 16:32

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Contributors

Author: M.W. Watson
Author: P.R. Lambden
Author: I.N. Clarke ORCID iD

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