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Genotyping of Chlamydia trachomatis from a trachoma-endemic village in the Gambia by a nested polymerase chain reaction: identification of strain variants

Genotyping of Chlamydia trachomatis from a trachoma-endemic village in the Gambia by a nested polymerase chain reaction: identification of strain variants
Genotyping of Chlamydia trachomatis from a trachoma-endemic village in the Gambia by a nested polymerase chain reaction: identification of strain variants
Direct amplification of the major outer membrane protein (MOMP) gene by polymerase chain reaction (PCR) was used to identify Chlamydia trachomatis in eye swabs from clinically active cases of endemic trachoma in a Gambian village. Chlamydial DNA was detected in 51% of 96 subjects with clinically active disease and in 5% of 37 clinically negative individuals. The PCR detection was combined with typing, using nested primers to variable sequences (VS) 1, 2, and 4 of the MOMP genes to distinguish between trachoma genotypes A, B, and C, respectively. Genotypes A and B were detected in the village, with some individuals harboring both genotypes within the same eye. DNA sequencing revealed strain variants of both genotypes. Typing of genotype and strain variants is now in progress to study trachoma transmission within the village.
0022-1899
1173-1177
Hayes, Lyn J.
37dc7661-d9b9-4605-be78-4c65b0a3237d
Bailey, Robin L.
ee2d7167-8ead-46f1-9255-f96aa7cc730b
Mabey, David C.W.
aad3d714-ad9e-43e5-9fd8-31437c8e9b10
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Pickett, Mark A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Watt, Peter J.
8a27f505-e0f4-4663-a19a-ef7f156c8863
Ward, Michael E.
a199bc96-75b6-415c-bffe-e68e8a00b468
Hayes, Lyn J.
37dc7661-d9b9-4605-be78-4c65b0a3237d
Bailey, Robin L.
ee2d7167-8ead-46f1-9255-f96aa7cc730b
Mabey, David C.W.
aad3d714-ad9e-43e5-9fd8-31437c8e9b10
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Pickett, Mark A.
4b358cb6-2f2e-4691-a314-0e80df48eadb
Watt, Peter J.
8a27f505-e0f4-4663-a19a-ef7f156c8863
Ward, Michael E.
a199bc96-75b6-415c-bffe-e68e8a00b468

Hayes, Lyn J., Bailey, Robin L., Mabey, David C.W., Clarke, Ian N., Pickett, Mark A., Watt, Peter J. and Ward, Michael E. (1992) Genotyping of Chlamydia trachomatis from a trachoma-endemic village in the Gambia by a nested polymerase chain reaction: identification of strain variants. The Journal of Infectious Diseases, 166 (5), 1173-1177. (doi:10.1093/infdis/166.5.1173). (PMID:1402030)

Record type: Article

Abstract

Direct amplification of the major outer membrane protein (MOMP) gene by polymerase chain reaction (PCR) was used to identify Chlamydia trachomatis in eye swabs from clinically active cases of endemic trachoma in a Gambian village. Chlamydial DNA was detected in 51% of 96 subjects with clinically active disease and in 5% of 37 clinically negative individuals. The PCR detection was combined with typing, using nested primers to variable sequences (VS) 1, 2, and 4 of the MOMP genes to distinguish between trachoma genotypes A, B, and C, respectively. Genotypes A and B were detected in the village, with some individuals harboring both genotypes within the same eye. DNA sequencing revealed strain variants of both genotypes. Typing of genotype and strain variants is now in progress to study trachoma transmission within the village.

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Published date: November 1992
Organisations: Faculty of Medicine

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Local EPrints ID: 352641
URI: https://eprints.soton.ac.uk/id/eprint/352641
ISSN: 0022-1899
PURE UUID: 557772fb-db2b-4b4c-ad41-cc2008a1feab
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 04 Jun 2013 12:58
Last modified: 06 Jun 2018 13:19

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Contributors

Author: Lyn J. Hayes
Author: Robin L. Bailey
Author: David C.W. Mabey
Author: Ian N. Clarke ORCID iD
Author: Mark A. Pickett
Author: Peter J. Watt
Author: Michael E. Ward

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