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The correct sequence of the porcine group C/Cowden rotavirus major inner capsid protein shows close homology with human isolates from Brazil and the U.K.

The correct sequence of the porcine group C/Cowden rotavirus major inner capsid protein shows close homology with human isolates from Brazil and the U.K.
The correct sequence of the porcine group C/Cowden rotavirus major inner capsid protein shows close homology with human isolates from Brazil and the U.K.
Amino acid sequence alignments between the human group C/Bristol and the published porcine group C/Cowden VP6 proteins have revealed a region of extreme sequence divergence. We have been unable to confirm the nucleotide sequence of the Cowden VP6 gene corresponding to this region of divergence. Direct sequencing of a PCR-amplified cDNA pool has revealed a frame shift, and three nucleotide changes, within the published sequence of the porcine (Cowden) VP6 gene. The corrected sequence of the porcine protein revealed a closer homology with VP6 from the Bristol strain and two new human group C rotavirus isolates. Atypical rotaviruses have been detected in the feces of children living in Belém, Brazil, and Preston, U.K. Direct sequencing of PCR-amplified cDNA corresponding to the VP6 gene of one isolate from each location confirmed the presence of a group C rotavirus. The complete nucleotide sequences of the VP6 genes from the group C/Belém and C/Preston rotaviruses contained an open reading frame of 1185 nucleotides (395 amino acids; deduced M(r) 44,669 Da). The Belém VP6 gene demonstrated 97.9% nucleotide homology with the human group C/Bristol VP6 gene and 83.4% nucleotide homology (91.6% deduced amino acid homology) with the corrected porcine group C/Cowden sequence. The Preston VP6 gene demonstrated 99.6% nucleotide homology with the human group C/Bristol VP6 gene and 84.0% nucleotide homology (91.6% deduced amino acid homology) with the corrected porcine group C/Cowden sequence. Remarkably, the deduced amino acid sequence of the Brazilian strain was identical to that of the U.K. isolates.
0042-6822
531-537
Cooke, S.J.
01ed572d-e621-4c90-be53-97ac1b1bfdcd
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Freitas, R.B.
e9e06487-3337-4708-90ec-3a6fffff23f8
Gabbay, Y.B.
a042a790-fa31-423d-aca3-b6144d5b96c5
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77
Cooke, S.J.
01ed572d-e621-4c90-be53-97ac1b1bfdcd
Clarke, I.N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Freitas, R.B.
e9e06487-3337-4708-90ec-3a6fffff23f8
Gabbay, Y.B.
a042a790-fa31-423d-aca3-b6144d5b96c5
Lambden, P.R.
e99ecc21-50d7-4a43-9e79-efba46592c77

Cooke, S.J., Clarke, I.N., Freitas, R.B., Gabbay, Y.B. and Lambden, P.R. (1992) The correct sequence of the porcine group C/Cowden rotavirus major inner capsid protein shows close homology with human isolates from Brazil and the U.K. Virology, 190 (1), 531-537. (doi:10.1016/0042-6822(92)91248-S). (PMID:1326817)

Record type: Article

Abstract

Amino acid sequence alignments between the human group C/Bristol and the published porcine group C/Cowden VP6 proteins have revealed a region of extreme sequence divergence. We have been unable to confirm the nucleotide sequence of the Cowden VP6 gene corresponding to this region of divergence. Direct sequencing of a PCR-amplified cDNA pool has revealed a frame shift, and three nucleotide changes, within the published sequence of the porcine (Cowden) VP6 gene. The corrected sequence of the porcine protein revealed a closer homology with VP6 from the Bristol strain and two new human group C rotavirus isolates. Atypical rotaviruses have been detected in the feces of children living in Belém, Brazil, and Preston, U.K. Direct sequencing of PCR-amplified cDNA corresponding to the VP6 gene of one isolate from each location confirmed the presence of a group C rotavirus. The complete nucleotide sequences of the VP6 genes from the group C/Belém and C/Preston rotaviruses contained an open reading frame of 1185 nucleotides (395 amino acids; deduced M(r) 44,669 Da). The Belém VP6 gene demonstrated 97.9% nucleotide homology with the human group C/Bristol VP6 gene and 83.4% nucleotide homology (91.6% deduced amino acid homology) with the corrected porcine group C/Cowden sequence. The Preston VP6 gene demonstrated 99.6% nucleotide homology with the human group C/Bristol VP6 gene and 84.0% nucleotide homology (91.6% deduced amino acid homology) with the corrected porcine group C/Cowden sequence. Remarkably, the deduced amino acid sequence of the Brazilian strain was identical to that of the U.K. isolates.

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Published date: September 1992
Organisations: Faculty of Medicine

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Local EPrints ID: 352642
URI: https://eprints.soton.ac.uk/id/eprint/352642
ISSN: 0042-6822
PURE UUID: 8630aaa6-37b4-47dc-8964-80ded15e9811
ORCID for I.N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 04 Jun 2013 12:43
Last modified: 06 Jun 2018 13:19

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Contributors

Author: S.J. Cooke
Author: I.N. Clarke ORCID iD
Author: R.B. Freitas
Author: Y.B. Gabbay
Author: P.R. Lambden

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