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Capsid diversity in small round-structured viruses: molecular characterization of an antigenically distinct human enteric calicivirus

Capsid diversity in small round-structured viruses: molecular characterization of an antigenically distinct human enteric calicivirus
Capsid diversity in small round-structured viruses: molecular characterization of an antigenically distinct human enteric calicivirus
Studies of antigenic variation between small round-structured viruses (SRSVs) using immune electron microscopy have revealed 3 antigenic types currently circulating in the UK represented by the strains SRSV/Bri/93/UK, SRSV/Sot/91/UK and SRSV/Mel/89/UK. Mel/89/UK RNA was isolated from a 1989 school outbreak of gastroenteritis. The 3'-terminal 3435 nucleotides (excluding the poly(A) tail) were determined by RT-PCR and cDNA sequencing, completing our molecular characterization of antigenically diverse SRSVs. Coding regions for the calicivirus RNA polymerase and capsid protein were found together with a 3' open reading frame of unknown function. The polymerase region was most highly conserved between Mel/89/UK and the other two SRSVs while the 3' open reading frame exhibited extreme variation. Phylogenetic analysis of SRSV capsids showed that Mel/89/UK differed significantly from Bri/93/UK and Sot/91/UK (62 and 39% identity, respectively) and was distinct from 6 other non-UK SRSVs that had been previously characterized. This was consistent with the designation of Mel/89/UK as a novel antigenic variant. Comparison of the capsid amino acid sequences of the 3 UK strains together with the antigenically distinct SRSV/Nor/68/US revealed a hypervariable region that could be surface-exposed and contain the SRSV antigenic determinants.
calicivirus, capsid variation, antigenic typing, cDNA sequencing
0168-1702
271-283
Green, Steve M.
c11018d2-e940-4424-a0e3-7d844e5d286e
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Caul, E. Owen
177391c9-26e2-4c94-a459-d9353c2daa61
Ashley, Charles R.
f5151218-e9e0-4bc5-9dfd-bac2b04a0e31
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Green, Steve M.
c11018d2-e940-4424-a0e3-7d844e5d286e
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Caul, E. Owen
177391c9-26e2-4c94-a459-d9353c2daa61
Ashley, Charles R.
f5151218-e9e0-4bc5-9dfd-bac2b04a0e31
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b

Green, Steve M., Lambden, Paul R., Caul, E. Owen, Ashley, Charles R. and Clarke, Ian N. (1995) Capsid diversity in small round-structured viruses: molecular characterization of an antigenically distinct human enteric calicivirus. Virus Research, 37 (3), 271-283. (doi:10.1016/0168-1702(95)00041-N). (PMID:8533462)

Record type: Article

Abstract

Studies of antigenic variation between small round-structured viruses (SRSVs) using immune electron microscopy have revealed 3 antigenic types currently circulating in the UK represented by the strains SRSV/Bri/93/UK, SRSV/Sot/91/UK and SRSV/Mel/89/UK. Mel/89/UK RNA was isolated from a 1989 school outbreak of gastroenteritis. The 3'-terminal 3435 nucleotides (excluding the poly(A) tail) were determined by RT-PCR and cDNA sequencing, completing our molecular characterization of antigenically diverse SRSVs. Coding regions for the calicivirus RNA polymerase and capsid protein were found together with a 3' open reading frame of unknown function. The polymerase region was most highly conserved between Mel/89/UK and the other two SRSVs while the 3' open reading frame exhibited extreme variation. Phylogenetic analysis of SRSV capsids showed that Mel/89/UK differed significantly from Bri/93/UK and Sot/91/UK (62 and 39% identity, respectively) and was distinct from 6 other non-UK SRSVs that had been previously characterized. This was consistent with the designation of Mel/89/UK as a novel antigenic variant. Comparison of the capsid amino acid sequences of the 3 UK strains together with the antigenically distinct SRSV/Nor/68/US revealed a hypervariable region that could be surface-exposed and contain the SRSV antigenic determinants.

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More information

Published date: August 1995
Keywords: calicivirus, capsid variation, antigenic typing, cDNA sequencing
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 352653
URI: https://eprints.soton.ac.uk/id/eprint/352653
ISSN: 0168-1702
PURE UUID: 6cb3cebf-6327-4371-8ab1-f69202934ceb
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

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Date deposited: 05 Jun 2013 11:00
Last modified: 06 Jun 2018 13:19

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Contributors

Author: Steve M. Green
Author: Paul R. Lambden
Author: E. Owen Caul
Author: Charles R. Ashley
Author: Ian N. Clarke ORCID iD

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